ArticleProceedings of the National Academy of Sciences of the United States of America2014
High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
106 citing papers in PubMed.
- DeepTRACE brings flexible machine learning to single-molecule track analysis.Communications biology · 2026Article
- Looking at bacterial cell poles from a liquid-liquid phase separation of intrinsically disordered proteins perspective.Current research in structural biology · 2025Article
- Crosslinking by ZapD drives the assembly of short FtsZ filaments into toroidal structures in solution.eLife · 2025Article
- Enabling real-time reconstruction for large field-of-view single-molecule localization microscopy using discrete field-dependent point-spread function.Biomedical optics express · 2025Article
- Article
- Determining the rate-limiting processes for cell division in Escherichia coli.Nature communications · 2024Article
- HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- BNP-Track: a framework for superresolved tracking.Nature methods · 2024Article
- Chromosome organization shapes replisome dynamics in Caulobacter crescentus.Nature communications · 2024Article
- An integrated platform for high-throughput nanoscopy.Nature biotechnology · 2023Article
- Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging.Nature methods · 2023Article
- Models versus pathogens: how conserved is the FtsZ in bacteria?Bioscience reports · 2023Review
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- Maximum-likelihood model fitting for quantitative analysis of SMLM data.Nature methods · 2023Article
- FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli.Nature communications · 2022Article
- Article
- FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division.Nature communications · 2021Article
- Predicting the membrane permeability of organic fluorescent probes by the deep neural network based lipophilicity descriptor DeepFl-LogP.Scientific reports · 2021Article
- Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.iScience · 2021Review
- Ectopic positioning of the cell division plane is associated with single amino acid substitutions in the FtsZ-recruiting SsgB inOpen biology · 2021Article
46 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.