ArticleProceedings of the National Academy of Sciences of the United States of America2014
Neuronal calcium-binding proteins 1/2 localize to dorsal root ganglia and excitatory spinal neurons and are regulated by nerve injury.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 58 citations in OpenAlex.
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- Early results of the integrative epigenomic-transcriptomic landscape of colorectal adenoma and cancer.World journal of gastrointestinal oncology · 2024Article
- Glucocorticoid receptor-NECAB1 axis can negatively regulate insulin secretion in pancreatic β-cells.Scientific reports · 2023Article
- Emergence and Progression of Behavioral Motor Deficits and Skeletal Muscle Atrophy across the Adult Lifespan of the Rat.Biology · 2023Article
- A neural tract tracing study on synaptic connections for cortical glutamatergic terminals and cervical spinal calretinin neurons in rats.Frontiers in neural circuits · 2023Article
- Intravital 3D visualization and segmentation of murine neural networks at micron resolution.Scientific reports · 2022Article
- Autism-Risk GeneFrontiers in molecular neuroscience · 2022Article
- NECAB1 and NECAB2 are Prevalent Calcium-Binding Proteins of CB1/CCK-Positive GABAergic Interneurons.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by Interacting with NECAB2.Journal of molecular neuroscience : MN · 2021Article
- Passive Clearing and 3D Lightsheet Imaging of the Intact and Injured Spinal Cord in Mice.Frontiers in cellular neuroscience · 2021Article
- Expression of huntingtin-associated protein 1 in adult mouse dorsal root ganglia and its neurochemical characterization in reference to sensory neuron subpopulations.IBRO reports · 2020Article
- Recent advances in our understanding of the organization of dorsal horn neuron populations and their contribution to cutaneous mechanical allodynia.Journal of neural transmission (Vienna, Austria : 1996) · 2020Review
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY.BioMed research international · 2020Article
- Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn.eLife · 2019Article
- Article
- Common Polymorphisms in Genes Related to Vitamin D Metabolism Affect the Response of Cognitive Abilities to Vitamin D Supplementation.Journal of molecular neuroscience : MN · 2019Article
- Ca2+-binding protein NECAB2 facilitates inflammatory pain hypersensitivity.The Journal of clinical investigation · 2018Article
- An innate immune response and altered nuclear receptor activation defines the spinal cord transcriptome during alpha-tocopherol deficiency in Ttpa-null mice.Free radical biology & medicine · 2018Article
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Authors and funding
15 authors at 6 institutions in 5 countries.
Funding
Abstract
Neuronal calcium (Ca(2+))-binding proteins 1 and 2 (NECAB1/2) are members of the phylogenetically conserved EF-hand Ca(2+)-binding protein superfamily. To date, NECABs have been explored only to a limited extent and, so far, not at all at the spinal level. Here, we describe the distribution, phenotype, and nerve injury-induced regulation of NECAB1/NECAB2 in mouse dorsal root ganglia (DRGs) and spinal cord. In DRGs, NECAB1/2 are expressed in around 70% of mainly small- and medium-sized neurons. Many colocalize with calcitonin gene-related peptide and isolectin B4, and thus represent nociceptors. NECAB1/2 neurons are much more abundant in DRGs than the Ca(2+)-binding proteins (parvalbumin, calbindin, calretinin, and secretagogin) studied to date. In the spinal cord, the NECAB1/2 distribution is mainly complementary. NECAB1 labels interneurons and a plexus of processes in superficial layers of the dorsal horn, commissural neurons in the intermediate area, and motor neurons in the ventral horn. Using CLARITY, a novel, bilaterally connected neuronal system with dendrites that embrace the dorsal columns like palisades is observed. NECAB2 is present in cell bodies and presynaptic boutons across the spinal cord. In the dorsal horn, most NECAB1/2 neurons are glutamatergic. Both NECAB1/2 are transported into dorsal roots and peripheral nerves. Peripheral nerve injury reduces NECAB2, but not NECAB1, expression in DRG neurons. Our study identifies NECAB1/2 as abundant Ca(2+)-binding proteins in pain-related DRG neurons and a variety of spinal systems, providing molecular markers for known and unknown neuron populations of mechanosensory and pain circuits in the spinal cord.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.