ArticleProceedings of the National Academy of Sciences of the United States of America2014
Structural dynamics of troponin during activation of skeletal muscle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Subsarcomeric regulation of thin and thick filaments in skeletal muscle myofibrils.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Calcium-binding proteins as allergens.Frontiers in allergy · 2026Review
- Structural changes in troponin during activation of skeletal and heart muscle determined in situ by polarised fluorescence.Biophysical reviews · 2024Review
- Dependence of myosin filament structure on intracellular calcium concentration in skeletal muscle.The Journal of general physiology · 2023Article
- Activation of skeletal muscle is controlled by a dual-filament mechano-sensing mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cooling intact and demembranated trabeculae from rat heart releases myosin motors from their inhibited conformation.The Journal of general physiology · 2022Article
- The regulatory light chain mediates inactivation of myosin motors during active shortening of cardiac muscle.Nature communications · 2021Article
- Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle.eLife · 2021Article
- Stress-dependent activation of myosin in the heart requires thin filament activation and thick filament mechanosensing.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dependence of thick filament structure in relaxed mammalian skeletal muscle on temperature and interfilament spacing.The Journal of general physiology · 2021Article
- Computationally efficient model of myocardial electromechanics for multiscale simulations.PloS one · 2021Article
- Myosin filament-based regulation of the dynamics of contraction in heart muscle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cycling Cross-Bridges Contribute to Thin Filament Activation in Human Slow-Twitch Fibers.Frontiers in physiology · 2020Article
- Titin-mediated thick filament activation stabilizes myofibrils on the descending limb of their force-length relationship.Journal of sport and health science · 2018Article
- Regulation of Contraction by the Thick Filaments in Skeletal Muscle.Biophysical journal · 2017Review
- The molecular basis of thin filament activation: from single molecule to muscle.Scientific reports · 2017Article
- Single molecule optical measurements of orientation and rotations of biological macromolecules.Methods and applications in fluorescence · 2016Article
- Thick filament mechano-sensing is a calcium-independent regulatory mechanism in skeletal muscle.Nature communications · 2016Article
- Order-Disorder Transitions in the Cardiac Troponin Complex.Journal of molecular biology · 2016Review
- Mechanosensing in Myosin Filament Solves a 60 Years Old Conflict in Skeletal Muscle Modeling between High Power Output and Slow Rise in Tension.Frontiers in physiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Time-resolved changes in the conformation of troponin in the thin filaments of skeletal muscle were followed during activation in situ by photolysis of caged calcium using bifunctional fluorescent probes in the regulatory and the coiled-coil (IT arm) domains of troponin. Three sequential steps in the activation mechanism were identified. The fastest step (1,100 s(-1)) matches the rate of Ca(2+) binding to the regulatory domain but also dominates the motion of the IT arm. The second step (120 s(-1)) coincides with the azimuthal motion of tropomyosin around the thin filament. The third step (15 s(-1)) was shown by three independent approaches to track myosin head binding to the thin filament, but is absent in the regulatory head. The results lead to a four-state structural kinetic model that describes the molecular mechanism of muscle activation in the thin filament-myosin head complex under physiological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.