ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2014
Improved quantitative analysis of primary bone marrow megakaryocytes utilizing imaging flow cytometry.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Translation of the Morphological Hallmarks of Dyserythropoiesis to Objective Morphometric Parameters by Imaging Flow Cytometry.International journal of laboratory hematology · 2025Article
- Beyond Thrombopoiesis: The Immune Functions of Megakaryocytes in Bacterial Infections and Sepsis.International journal of molecular sciences · 2025Review
- Highly Efficient Calibration-Free Color Compensation Algorithm for Imaging Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2025Article
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- Long-lived lung megakaryocytes contribute to platelet recovery in thrombocytopenia models.The Journal of clinical investigation · 2024Article
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- Long-acting PGE2 and Lisinopril Mitigate H-ARS.Radiation research · 2021Article
- Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis.Epigenetics & chromatin · 2021Article
- Acute and late effects of combined internal and external radiation exposures on the hematopoietic system.International journal of radiation biology · 2019Article
- Analysis of procoagulant phosphatidylserine-exposing platelets by imaging flow cytometry.Research and practice in thrombosis and haemostasis · 2018Article
- Quantification and three-dimensional microanatomical organization of the bone marrow.Blood advances · 2017Review
- Is There a Role for Flow Cytometry in the Evaluation of Patients With Myelodysplastic Syndromes?Current hematologic malignancy reports · 2015Review
- Utilization of imaging flow cytometry to define intermediates of megakaryopoiesis in vivo and in vitro.Journal of immunological methods · 2015Article
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4 authors at 1 institution in 1 country.
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Abstract
Life-threatening thrombocytopenia can develop following bone marrow injury due to decreased platelet production from megakaryocytes (MKs). However, the study of primary MKs has been complicated by their low frequency in the bone marrow and by technical challenges presented by their unique maturation properties. More accurate and efficient methods for the analysis of in vivo MKs are needed to enhance our understanding of megakaryopoiesis and ultimately develop new therapeutic strategies for thrombocytopenia. Imaging flow cytometry (IFC) combines the morphometric capabilities of microscopy with the high-throughput analyses of flow cytometry (FC). Here, we investigate the application of IFC on the ImageStream(X) platform to the analysis of primary MKs isolated from murine bone marrow. Our data highlight and address technical challenges for conventional FC posed by the wide range of cellular size within the MK lineage as well as the shared surface phenotype with abundant platelet progeny. We further demonstrate that IFC can be used to reproducibly and efficiently quantify the frequency of primary murine MKs in the marrow, both at steady-state and in the setting of radiation-induced bone marrow injury, as well as assess their ploidy distribution. The ability to accurately analyze the full spectrum of maturing MKs in the bone marrow now allows for many possible applications of IFC to enhance our understanding of megakaryopoiesis and platelet production.
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