ArticleChembiochem : a European journal of chemical biology2014
Rapid total synthesis of DARPin pE59 and barnase.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
- Aspartimide Formation and Its Prevention in Fmoc Chemistry Solid Phase Peptide Synthesis.Chembiochem : a European journal of chemical biology · 2025Review
- Backbone Protecting Groups for Enhanced Peptide and Protein Synthesis.Angewandte Chemie (International ed. in English) · 2025Review
- Amide Bonds Meet Flow Chemistry: A Journey into Methodologies and Sustainable Evolution.ChemSusChem · 2022Review
- Synthesis, Characterization and Evaluation of Peptide Nanostructures for Biomedical Applications.Molecules (Basel, Switzerland) · 2021Review
- Greening the synthesis of peptide therapeutics: an industrial perspective.RSC advances · 2020Review
- Automated Flow Synthesis of Tumor Neoantigen Peptides for Personalized Immunotherapy.Scientific reports · 2020Article
- Heterochiral Knottin Protein: Folding and Solution Structure.Biochemistry · 2017Article
- A fully automated flow-based approach for accelerated peptide synthesis.Nature chemical biology · 2017Article
- A Facile N-Mercaptoethoxyglycinamide (MEGA) Linker Approach to Peptide Thioesterification and Cyclization.Journal of the American Chemical Society · 2017Article
- Serine promoted synthesis of peptide thioester-precursor on solid support for native chemical ligation.Chemical science · 2017Article
- Total chemical synthesis of a thermostable enzyme capable of polymerase chain reaction.Cell discovery · 2017Article
- Total chemical synthesis, refolding, and crystallographic structure of fully active immunophilin calstabin 2 (FKBP12.6).Protein science : a publication of the Protein Society · 2016Article
- Article
- Total synthesis and biochemical characterization of mirror image barnase.Chemical science · 2015Article
- Directed evolution of multivalent glycopeptides tightly recognized by HIV antibody 2G12.Journal of the American Chemical Society · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We report the convergent total synthesis of two proteins: DARPin pE59 and Bacillus amyloliquefaciens RNase (Barnase). Leveraging our recently developed fast-flow peptide-synthesis platform, we rapidly explored numerous conditions for the assembly of long polypeptides, and were able to mitigate common side reactions, including deletion and aspartimide products. We report general strategies for improving the synthetic quality of difficult peptide sequences with our system. High-quality protein fragments produced under optimal synthetic conditions were subjected to convergent native chemical ligation, which afforded native full-length proteins after a final desulfurization step. Both DARPin and Barnase were folded and found to be as active as their recombinant analogues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.