ArticleThe Journal of cell biology2014
Bleb-driven chemotaxis of Dictyostelium cells.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
72 citing papers in PubMed, 109 citations in OpenAlex.
- Article
- Reconstitution of actomyosin networks in cell-sized liposomes dissects distinct mechanisms of membrane blebbing and symmetry breaking.Science advances · 2026Article
- Chemical and Mechanical Regulation of Leukocyte Migration.Cold Spring Harbor perspectives in biology · 2026Review
- A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives from Dictyostelium discoideum.BMC molecular and cell biology · 2026Article
- Chemotactic motion of coronin-null cells with impaired actin turnover.BMC molecular and cell biology · 2026Article
- A role for PaxB in regulating blebbing: experimental insights and theoretical perspectives fromResearch square · 2026Article
- Article
- Signaling and actin waves at a glance.Journal of cell science · 2025Review
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Reconstitution of actomyosin networks in cell-sized liposomes reveals distinct mechanical roles of cytoskeletal organization in membrane shape remodeling.bioRxiv : the preprint server for biology · 2025Article
- Effects of nocodazole and latrunculin B on locomotion of amoeboid cells of Rhizochromulina sp. strain B44 (Heterokontophyta, Dictyochophyceae).Protoplasma · 2025Article
- Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration.Development (Cambridge, England) · 2025Article
- The IQGAP-related RasGAP IqgC regulates cell-substratum adhesion in Dictyostelium discoideum.Cellular & molecular biology letters · 2025Article
- Blebology: principles of bleb-based migration.Trends in cell biology · 2024Review
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- Ras suppression potentiates rear actomyosin contractility-driven cell polarization and migration.Nature cell biology · 2024Article
- GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3.The Journal of cell biology · 2023Article
- Centrosome Positioning in MigratingCells · 2022Article
- A reduced 1D stochastic model of bleb-driven cell migration.Biophysical journal · 2022Article
- Blebs-Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration.Frontiers in cell and developmental biology · 2022Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Blebs and F-actin-driven pseudopods are alternative ways of extending the leading edge of migrating cells. We show that Dictyostelium cells switch from using predominantly pseudopods to blebs when migrating under agarose overlays of increasing stiffness. Blebs expand faster than pseudopods leaving behind F-actin scars, but are less persistent. Blebbing cells are strongly chemotactic to cyclic-AMP, producing nearly all of their blebs up-gradient. When cells re-orientate to a needle releasing cyclic-AMP, they stereotypically produce first microspikes, then blebs and pseudopods only later. Genetically, blebbing requires myosin-II and increases when actin polymerization or cortical function is impaired. Cyclic-AMP induces transient blebbing independently of much of the known chemotactic signal transduction machinery, but involving PI3-kinase and downstream PH domain proteins, CRAC and PhdA. Impairment of this PI3-kinase pathway results in slow movement under agarose and cells that produce few blebs, though actin polymerization appears unaffected. We propose that mechanical resistance induces bleb-driven movement in Dictyostelium, which is chemotactic and controlled through PI3-kinase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.