ArticleFunctional & integrative genomics2014
Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco.
Article in Functional & integrative genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Genome-wide molecular characterization and expression profiling of the cysteine protease gene family in maize.BMC genomics · 2025Article
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus.Plants (Basel, Switzerland) · 2023Article
- MYB2 Is Important for Tapetal PCD and Pollen Development by Directly Activating Protease Expression inInternational journal of molecular sciences · 2022Article
- Genome-wide association identifies candidate genes for drought tolerance in coast redwood and giant sequoia.The Plant journal : for cell and molecular biology · 2022Article
- Genome-wide comparative transcriptome analysis of the A4-CMS line ICPA 2043 and its maintainer ICPB 2043 during the floral bud development of pigeonpea.Functional & integrative genomics · 2021Article
- Male Sterility is linked to the Flavonoid Biosynthesis Pathways in Prunus mira.Bioinformation · 2020Article
- Targeted expression of a cysteine protease (AdCP) in tapetum induces male sterility in Indian mustard, Brassica juncea.Functional & integrative genomics · 2019Article
- A proteomic study of cysteine protease induced cell death in anthers of male sterile tobacco transgenic plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Evolvement of transgenic male-sterility and fertility-restoration system in rice for production of hybrid varieties.Plant molecular biology · 2018Article
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- Molecular Approaches for Manipulating Male Sterility and Strategies for Fertility Restoration in Plants.Molecular biotechnology · 2017Review
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Usable male sterility systems have immense potential in developing hybrid varieties in crop plants, which can also be used as a biological safety containment to prevent horizontal transgene flow. Barnase-Barstar system developed earlier was the first approach to engineer male sterility in plants. In an analogous situation, we have evolved a system of inducing pollen abortion and male sterility in transgenic tobacco by expressing a plant gene coding for a protein with known developmental function in contrast to the Barnase-Barstar system, which deploys genes of prokaryotic origin, i.e., from Bacillus amyloliquefaciens. We have used a plant pathogen-induced gene, cysteine protease for inducing male sterility. This gene was identified in the wild peanut, Arachis diogoi differentially expressed when it was challenged with the late leaf spot pathogen, Phaeoisariopsis personata. Arachis diogoi cysteine protease (AdCP) was expressed under the strong tapetum-specific promoter (TA29) and tobacco transformants were generated. Morphological and histological analysis of AdCP transgenic plants showed ablated tapetum and complete pollen abortion in three transgenic lines. Furthermore, transcript analysis displayed the expression of cysteine protease in these male sterile lines and the expression of the protein was identified in western blot analysis using its polyclonal antibody raised in the rabbit system.
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