ArticleInfection and immunity2014
Establishment of three Francisella infections in zebrafish embryos at different temperatures.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
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- The Use of Extracellular Membrane Vesicles for Immunization against Francisellosis in Nile Tilapia (Vaccines · 2021Article
- Animal infection models using non-mammals.Microbiology and immunology · 2020Review
- DNA-based fluorescent probes of NOS2 activity in live brains.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review of diseases and health management in zebrafish Danio rerio (Hamilton 1822) in research facilities.Journal of fish diseases · 2020Review
- Larva of greater wax moth Galleria mellonella is a suitable alternative host for the fish pathogen Francisella noatunensis subsp. orientalis.BMC microbiology · 2020Article
- Zebrafish as an alternative animal model in human and animal vaccination research.Laboratory animal research · 2020Review
- Dual Transcriptomics of Host-Pathogen Interaction of Cystic Fibrosis IsolateFrontiers in cellular and infection microbiology · 2018Article
- Mecp2 regulatesDisease models & mechanisms · 2017Article
- Characterization and Vaccine Potential of Membrane Vesicles Produced by Francisella noatunensis subsp. orientalis in an Adult Zebrafish Model.Clinical and vaccine immunology : CVI · 2017Article
- Comparative Analysis of Membrane Vesicles from Three Piscirickettsia salmonis Isolates Reveals Differences in Vesicle Characteristics.PloS one · 2016Article
- Establishment of Infection Models in Zebrafish Larvae (Danio rerio) to Study the Pathogenesis of Aeromonas hydrophila.Frontiers in microbiology · 2016Article
- Dissection of Francisella-Host Cell Interactions in Dictyostelium discoideum.Applied and environmental microbiology · 2015Article
- Neutrophils in host defense: new insights from zebrafish.Journal of leukocyte biology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Francisella spp. are facultative intracellular pathogens identified in increasingly diverse hosts, including mammals. F. noatunensis subsp. orientalis and F. noatunensis subsp. noatunensis infect fish inhabiting warm and cold waters, respectively, while F. tularensis subsp. novicida is highly infectious for mice and has been widely used as a model for the human pathogen F. tularensis. Here, we established zebrafish embryo infection models of fluorescently labeled F. noatunensis subsp. noatunensis, F. noatunensis subsp. orientalis, and F. tularensis subsp. novicida at 22, 28, and 32°C, respectively. All infections led to significant bacterial growth, as shown by reverse transcription-quantitative PCR (RT-qPCR), and to a robust proinflammatory immune response, dominated by increased transcription of tumor necrosis factor alpha (TNF-α) and interleukin-1β (IL-1β). F. noatunensis subsp. orientalis was the most virulent, F. noatunensis subsp. noatunensis caused chronic infection, and F. tularensis subsp. novicida showed moderate virulence and led to formation of relatively small granuloma-like structures. The use of transgenic zebrafish strains with enhanced green fluorescent protein (EGFP)-labeled immune cells revealed their detailed interactions with Francisella species. All three strains entered preferentially into macrophages, which eventually assembled into granuloma-like structures. Entry into neutrophils was also observed, though the efficiency of this event depended on the route of infection. The results demonstrate the usefulness of the zebrafish embryo model for studying infections caused by different Francisella species at a wide range of temperatures and highlight their interactions with immune cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.