ArticleInfection and immunity2014
Differential requirement for PBP1a and PBP1b in in vivo and in vitro fitness of Vibrio cholerae.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Clinical characteristics, specific resistance patterns, and molecular mechanisms of carbapenem-resistantFrontiers in cellular and infection microbiology · 2025Article
- Mslar: Microbial synthetic lethal and rescue database.PLoS computational biology · 2023Review
- Recovery of Vibrio cholerae polarized cellular organization after exit from a non-proliferating spheroplast state.PloS one · 2023Article
- Transposon insertion site sequencing (TIS) of Pseudomonas aeruginosa.Journal of microbiology (Seoul, Korea) · 2021Article
- Understanding tolerance to cell wall-active antibiotics.Annals of the New York Academy of Sciences · 2021Review
- CrvA and CrvB form a curvature-inducing module sufficient to induce cell-shape complexity in Gram-negative bacteria.Nature microbiology · 2021Article
- Article
- A multifaceted cellular damage repair and prevention pathway promotes high-level tolerance to β-lactam antibiotics.EMBO reports · 2021Article
- Genetic analysis of the role of the conserved inner membrane protein CvpA in EHEC resistance to deoxycholate.Journal of bacteriology · 2020Article
- Review
- Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides.Cell reports · 2020Article
- Article
- Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli.The EMBO journal · 2020Article
- Double Tubular Contractile Structure of the Type VI Secretion System Displays Striking Flexibility and Elasticity.Journal of bacteriology · 2019Article
- Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.PLoS pathogens · 2019Article
- A Vibrio cholerae BolA-Like Protein Is Required for Proper Cell Shape and Cell Envelope Integrity.mBio · 2019Article
- Simultaneously inhibiting undecaprenyl phosphate production and peptidoglycan synthases promotes rapid lysis in Escherichia coli.Molecular microbiology · 2019Article
- Unsupervised Learning Approach for Comparing Multiple Transposon Insertion Sequencing Studies.mSphere · 2019Article
- Interplay between Peptidoglycan Biology and Virulence in Gram-Negative Pathogens.Microbiology and molecular biology reviews : MMBR · 2018Review
- Genetic Determinants of Penicillin Tolerance in Vibrio cholerae.Antimicrobial agents and chemotherapy · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
We investigated the roles of the Vibrio cholerae high-molecular-weight bifunctional penicillin binding proteins, PBP1a and PBP1b, in the fitness of this enteric pathogen. Using a screen for synthetic lethality, we found that the V. cholerae PBP1a and PBP1b proteins, like their Escherichia coli homologues, are each essential in the absence of the other and in the absence of the other's putative activator, the outer membrane lipoproteins LpoA and LpoB, respectively. Comparative analyses of V. cholerae mutants suggest that PBP1a/LpoA of V. cholerae play a more prominent role in generating and/or maintaining the pathogen's cell wall than PBP1b/LpoB. V. cholerae lacking PBP1b or LpoB exhibited wild-type growth under all conditions tested. In contrast, V. cholerae lacking PBP1a or LpoA exhibited growth deficiencies in minimal medium, in the presence of deoxycholate and bile, and in competition assays with wild-type cells both in vitro and in the infant mouse small intestine. PBP1a pathway mutants are particularly impaired in stationary phase, which renders them sensitive to a product(s) present in supernatants from stationary-phase wild-type cells. The marked competitive defect of the PBP1a pathway mutants in vivo was largely absent when exponential-phase cells rather than stationary-phase cells were used to inoculate suckling mice. Thus, at least for V. cholerae PBP1a pathway mutants, the growth phase of the inoculum is a key modulator of infectivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.