ArticlePloS one2014
Hope for restoration of dead valuable bulls through cloning using donor somatic cells isolated from cryopreserved semen.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- Comparative Evaluation of Methods of DNA Extraction from Cryopreserved Bovine Semen for Molecular Diagnostic Applications.Methods and protocols · 2026Article
- SCNT and genome editing in Buffalo, the black gold of the tropics.The Journal of reproduction and development · 2026Review
- Production of Water Buffalo SCNT Embryos by Handmade Cloning.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization.Scientific reports · 2022Article
- Article
- Isolation, culture, and characterization of cells derived from giant panda (Ailuropoda melanoleuca) semen.In vitro cellular & developmental biology. Animal · 2021Article
- Semen parameters and fertility potency of a cloned water buffalo (Bubalus bubalis) bull produced from a semen-derived epithelial cell.PloS one · 2020Article
- Successful cloning of a superior buffalo bull.Scientific reports · 2019Article
- Cloning of breeding buffalo bulls in India: Initiatives & challenges.The Indian journal of medical research · 2018Review
- Production of hand-made cloned buffalo (Bubalus bubalis) embryos from non-viable somatic cells.In vitro cellular & developmental biology. Animal · 2016Article
- Advances in reproductive biotechnologies.Veterinary world · 2016Review
- Production of a Cloned Buffalo (Bubalus bubalis) Calf from Somatic Cells Isolated from Urine.Cellular reprogramming · 2015Article
- Downregulation of DNA methyltransferase 1 in zona-free cloned buffalo (Bubalus bubalis) embryos by small interefering RNA improves in vitro development but does not alter DNA methylation level.Cellular reprogramming · 2015Article
- Article
- Article
- Addition of D-penicillamine, hypotaurine, and epinephrine (PHE) mixture to IVF medium maintains motility and longevity of bovine sperm and enhances stable production of blastocysts in vitro.The Journal of reproduction and development · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apoptotic index, were comparable among embryos produced using cells obtained from fresh or frozen-thawed semen or those obtained from conventional cell sources such as skin. Examination of the epigenetic status revealed that the global level of H3K27me3 but not that of H3K9/14ac and H4K5ac differed significantly (P<0.05) among cloned embryos from different bulls. The relative mRNA abundance of HDAC1, DNMT1, P53 and CASPASE 3 but not that of DNMT3a differed in cells and in cloned embryos. Following transfer of 24 cloned embryos produced from fresh semen-derived cells to 12 recipients, one calf weighing 55 kg, which is now 6 months of age and is normal, was born through normal parturition. Following transfer of 20 embryos produced from frozen-thawed semen-derived cells to 10 recipients, 2 became pregnant, one of which aborted in the first trimester; the calf born was severely underweight (17 kg), and died 12 h after birth. The ability of cells derived from fresh and frozen-thawed semen to produce live offspring confirms the ability of these cells to be reprogrammed. Our findings pave the way for restoration of highly precious progeny-tested bulls, which has immense economic importance, and can also be used for restoration of endangered species.
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