ArticleAntimicrobial agents and chemotherapy2014
Quantification of cell-associated atazanavir, darunavir, lopinavir, ritonavir, and efavirenz concentrations in human mononuclear cell extracts.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Pharmacokinetic approaches to standardize antiviral exposure in cerebrospinal fluid.Pharmacotherapy · 2025Article
- Establishment of a Four-Cell In Vitro Blood-Brain Barrier Model With Human Primary Brain Cells.Current protocols · 2024Article
- The anti-HIV drug abacavir stimulates β-catenin activity in osteoblast lineage cells.JBMR plus · 2024Article
- Quantification of nine antiretroviral drugs in cerebrospinal fluid: An approach to overcome sample collection tube adsorption.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023Article
- A novel 4-cell in-vitro blood-brain barrier model and its characterization by confocal microscopy and TEER measurement.Journal of neuroscience methods · 2023Article
- Article
- Assessing the lymphoid tissue bioavailability of antiretrovirals in human primary lymphoid endothelial cells and in mice.The Journal of antimicrobial chemotherapy · 2019Article
- Impact of Integrase Inhibition Compared With Nonnucleoside Inhibition on HIV Reservoirs in Lymphoid Tissues.Journal of acquired immune deficiency syndromes (1999) · 2019Article
- Adipocytes impair efficacy of antiretroviral therapy.Antiviral research · 2018Article
- Transplantation of CCR5∆32 Homozygous Umbilical Cord Blood in a Child With Acute Lymphoblastic Leukemia and Perinatally Acquired HIV Infection.Open forum infectious diseases · 2018Article
- Defining total-body AIDS-virus burden with implications for curative strategies.Nature medicine · 2017Article
- A Versatile Method to Determine the Cellular Bioavailability of Small-Molecule Inhibitors.Journal of medicinal chemistry · 2017Article
- Alterations in P-Glycoprotein Expression and Function Between Macrophage Subsets.Pharmaceutical research · 2016Article
- Mass spectrometry imaging reveals heterogeneous efavirenz distribution within putative HIV reservoirs.Antimicrobial agents and chemotherapy · 2015Article
- A rapid spin through oil results in higher cell-associated concentrations of antiretrovirals compared with conventional cell washing.Bioanalysis · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
An ultrasensitive assay utilizing high-pressure liquid chromatography and mass spectrometry detection was developed and validated for the quantification of the antiretrovirals atazanavir (ATV), darunavir (DRV), lopinavir (LPV), ritonavir (RTV), and efavirenz (EFV) in human mononuclear cell (MNC) extracts. The assay utilizes 20 μl of cellular extract that contains as few as 50,000 MNCs. The analytical range of the assay is 0.0200 to 10.0 fmol/μl for ATV, 0.0500 to 25.0 fmol/μl for DRV, LPV, and RTV, and 0.200 to 100 fmol/μl for EFV. The assay has proven to be a clinically useful tool for investigating antiretroviral drug concentrations in virologic sanctuaries where harvested cell numbers are extremely low. The assay provides a tool for investigators to explore the clinical pharmacology of strategies for prevention, treatment, and cure in pathophysiologically relevant sites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.