ArticlePloS one2014
Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom Agaricus bisporus.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 47 citations in OpenAlex.
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- AdvancingBioengineering (Basel, Switzerland) · 2024Review
- Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation.Applied microbiology and biotechnology · 2023Article
- Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity.iScience · 2023Article
- An in vitro method for inducing titan cells reveals novel features of yeast-to-titan switching in the human fungal pathogen Cryptococcus gattii.PLoS pathogens · 2022Article
- Comparative Transcriptome Analysis Provides Insights Into the Mechanism by Which 2,4-Dichlorophenoxyacetic Acid Improves Thermotolerance inFrontiers in microbiology · 2022Article
- Heat stress in macrofungi: effects and response mechanisms.Applied microbiology and biotechnology · 2021Review
- Transcriptional response of mushrooms to artificial sun exposure.Ecology and evolution · 2021Article
- Synthesis and Antibacterial Activity of New Azole, Diazole and Triazole Derivatives Based onMolecules (Basel, Switzerland) · 2021Article
- Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation.Scientific reports · 2021Article
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- Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance ofFrontiers in microbiology · 2020Article
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- Heat Stress Modulates Mycelium Growth, Heat Shock Protein Expression, Ganoderic Acid Biosynthesis, and Hyphal Branching of Ganoderma lucidum via Cytosolic Ca2.Applied and environmental microbiology · 2016Article
- Current technologies and related issues for mushroom transformation.Mycobiology · 2015Review
- Synthesis, crystal structure and biological activity of 2-hydroxyethylammonium salt of p-aminobenzoic acid.PloS one · 2014Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermo-sensitive. Here, we compared their responses at proteomic level to heat treatment at 33°C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e.g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.
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