Evidence map›Paper›PMID 24613086›Full record

ArticleThe Journal of nutritional biochemistry2014

Characterization of docosahexaenoic acid (DHA)-induced heme oxygenase-1 (HO-1) expression in human cancer cells: the importance of enhanced BTB and CNC homology 1 (Bach1) degradation.

Shuai Wang, Bethany N Hannafon, Roman F Wolf, Jundong Zhou, Jori E Avery, Jinchang Wu, Stuart E Lind, Wei-Qun Ding

Abstract read
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In one paragraph

Article in The Journal of nutritional biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Shuai WangDepartment of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 411A, Oklahoma City, OK 73104, USA.
Bethany N HannafonDepartment of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 411A, Oklahoma City, OK 73104, USA.
Roman F WolfDepartment of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 411A, Oklahoma City, OK 73104, USA.
Jundong ZhouDepartment of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, P. R. China.
Jori E AveryDepartment of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 411A, Oklahoma City, OK 73104, USA.
Jinchang WuDepartment of Radio-Oncology, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou, P. R. China.
Stuart E LindDepartments of Pathology and Medicine, University of Colorado School of Medicine, Aurora, CO, USA.
Wei-Qun DingDepartment of Pathology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 411A, Oklahoma City, OK 73104, USA. Electronic address: weiqun-ding@ouhsc.edu.
University of Oklahoma Health Sciences Center · USNanjing Medical University · CNUniversity of Colorado Denver · US

Funding

Tracking and Evaluation CoreU54GM104938 · NIGMS · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI Robert Hal Scofield · 2013 to 2026
$68.2M
VACCINE-TARGETED IDENTIFICATION OF FITNESS DETERMINANTS IN THE HIV-1 ENVELOPEP20RR016478 · NCRR · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI JIANG, YUHAO · 2001 to 2011
$35.8M
NCRR NIH HHS 3P20RR016478-09S2NCRR NIH HHS P20 RR016478NIGMS NIH HHS U54 GM104938
6 · The paper itself

Abstract

The effect of docosahexaenoic acid (DHA) on heme oxygenase-1 (HO-1) expression in cancer cells has never been characterized. This study examines DHA-induced HO-1 expression in human cancer cell model systems. DHA enhanced HO-1 gene expression in a time- and concentration-dependent manner, with maximal induction at 21 h of treatment. This induction of HO-1 expression was confirmed in vivo using a xenograft nude mouse model fed a fish-oil-enriched diet. The increase in HO-1 gene transcription induced by DHA was significantly attenuated by the antioxidant N-acetyl cysteine, suggesting the involvement of oxidative stress. This was supported by direct measurement of lipid peroxide levels after DHA treatment. Using a human HO-1 gene promoter reporter construct, we identified two antioxidant response elements (AREs) that mediate the DHA-induced increase in HO-1 gene transcription. Knockdown of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression compromised the DHA-induced increase in HO-1 gene transcription, indicating the importance of the Nrf2 pathway in this event. However, the nuclear protein levels of Nrf2 remained unchanged upon DHA treatment. Further studies demonstrated that DHA reduces nuclear Bach1 protein expression by promoting its degradation and attenuates Bach1 binding to the AREs in the HO-1 gene promoter. In contrast, DHA enhanced Nrf2 binding to the AREs without affecting nuclear Nrf2 expression levels, indicating a new cellular mechanism that mediates DHA's induction of HO-1 gene transcription. To our knowledge, this is the first characterization of DHA-induced HO-1 expression in human malignant cells.

Indexed as

AnimalsAntioxidant Response ElementsBasic-Leucine Zipper Transcription FactorsCell Line, TumorDocosahexaenoic AcidsFanconi Anemia Complementation Group ProteinsFemaleGene Expression Regulation, NeoplasticHeme Oxygenase-1HumansLipid PeroxidationMice, NudeNF-E2-Related Factor 2Promoter Regions, GeneticXenograft Model Antitumor AssaysBACH1 protein, humanBasic-Leucine Zipper Transcription FactorsDocosahexaenoic AcidsFanconi Anemia Complementation Group ProteinsHeme Oxygenase-1HMOX1 protein, humanNFE2L2 protein, humanNF-E2-Related Factor 2Antioxidant response elementCancerGene transcription regulationHeme oxygenase-1n-3 PUFA

Identifiers

PMID24613086
PMCPMC3988241
OpenAlexW1964235217

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.