ArticleChemistry & biology2014
MRI-based detection of alkaline phosphatase gene reporter activity using a porphyrin solubility switch.
Article in Chemistry & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- A molecular probe for detecting cholinesterase activity in MRI.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Water-Soluble Mn(III)-Porphyrins with High Relaxivity and Photosensitization.Chemical & biomedical imaging · 2025Article
- Review
- NMR Hyperpolarization Techniques of Gases.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Review
- An enzyme-activatable and cell-permeable MnChemical science · 2016Article
- High magnetic field induced otolith fusion in the zebrafish larvae.Scientific reports · 2016Article
- Development of Timd2 as a reporter gene for MRI.Magnetic resonance in medicine · 2016Article
- Tumor-specific expression and detection of a CEST reporter gene.Magnetic resonance in medicine · 2015Article
- Advances in using MRI probes and sensors for in vivo cell tracking as applied to regenerative medicine.Disease models & mechanisms · 2015Review
- Stimuli-responsive nanomaterials for biomedical applications.Journal of the American Chemical Society · 2015Article
- Microwave-assisted synthesis of [Nuclear medicine and biologyArticle
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The ability to map patterns of gene expression noninvasively in living animals could have impact in many areas of biology. Reporter systems compatible with MRI could be particularly valuable, but existing strategies tend to lack sensitivity or specificity. Here we address the challenge of MRI-based gene mapping using the reporter enzyme secreted alkaline phosphatase (SEAP), in conjunction with a water-soluble metalloporphyrin contrast agent. SEAP cleaves the porphyrin into an insoluble product that accumulates at sites of enzyme expression and can be visualized by MRI and optical absorbance. The contrast mechanism functions in vitro, in brain slices, and in animals. The system also provides the possibility of readout both in the living animal and by postmortem histology, and it notably does not require intracellular delivery of the contrast agent. The solubility switch mechanism used to detect SEAP could be adapted for imaging of additional reporter enzymes or endogenous targets.
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Registered trials
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