Evidence map›Paper›PMID 24613014›Full record

ArticleProstaglandins, leukotrienes, and essential fatty acids2014

Prostaglandin E2 modifies SMAD2 and promotes SMAD2-SMAD4 complex formation.

Chen Yang, Chen Chen, Andrey Sorokin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Prostaglandins, leukotrienes, and essential fatty acids, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Activation of the prostaglandin EActa physiologica (Oxford, England) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Chen YangDivision of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Chen ChenDepartment of Microbiology and Molecular Genetics, 8701 Watertown Plank Road, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Andrey SorokinDivision of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. Electronic address: sorokin@mcw.edu.
Medical College of Wisconsin · US

Funding

Endothelin Signaling and Actions in Renal MesangiumR01DK098159 · NIDDK · MEDICAL COLLEGE OF WISCONSIN · PI SOROKIN, ANDREY · 2013 to 2016
$1.3M
BK Virus Receptor and polyomavirus nephropathyR21DK088018 · NIDDK · MEDICAL COLLEGE OF WISCONSIN · PI SOROKIN, ANDREY · 2011 to 2012
$421k
NIDDK NIH HHS 1R01DK098159NIDDK NIH HHS 1R21DK088018NIDDK NIH HHS R01 DK098159NIDDK NIH HHS R21 DK088018
6 · The paper itself

Abstract

We report that PGE2 promotes Smad2-Smad4 complex formation and this phenomenon could be blocked by DIDS, an anion transporter inhibitor. Our data suggest that PGE2 had no effects on Smad2 phosphorylation, suggesting that PGE2-mediated Smad2-Smad4 complex formation is independent of TGF-β signaling and that PGE2 induced Smad2 modification which is different from TGF-β-mediated phosphorylation. We demonstrate that in primary human glomerular mesangial cells PGE2 caused modification of Smad2 as detected by Smad2N antibody, raised against a peptide near the N-terminus of Smad2. We hypothesize that Smad2 protein is post-translationaly modified by PGE2. Direct evidence of Smad2 modification by PGE2 was achieved by avidin pulldown assay which showed that endogenous Smad2 and recombinant Smad2 protein were attached by biotin-labeled PGE2. Taken together, our results provided evidence that post-translational modification of Smad2 could be a mechanism for the action of PGE2 in the pathogenesis of human pathologies.

Indexed as

Cells, CulturedDinoprostoneHumansMesangial CellsMultiprotein ComplexesProtein Processing, Post-TranslationalSignal TransductionSmad2 ProteinSmad4 ProteinTransforming Growth Factor betaDinoprostoneMultiprotein ComplexesSmad2 ProteinSMAD2 protein, humanSmad4 ProteinSMAD4 protein, humanTransforming Growth Factor betaMesangial cellsProstaglandinsSignaling

Identifiers

PMID24613014
PMCPMC4036222
OpenAlexW2084608962

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.