ArticleBMC genomics2014
Target enrichment using parallel nanoliter quantitative PCR amplification.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Mechanism of microbial action of the inoculated nitrogen-fixing bacterium for growth promotion and yield enhancement in rice (Oryza sativa L.).Advanced biotechnology · 2024Article
- Genetic Diversity of MHC B-F/B-L Region in 21 Chicken Populations.Frontiers in genetics · 2021Article
- Construction and forensic application of 20 highly polymorphic microhaplotypes.Royal Society open science · 2020Article
- Fusing MEMS technology with lab-on-chip: nanoliter-scale silicon microcavity arrays for digital DNA quantification and multiplex testing.Microsystems & nanoengineering · 2020Article
- Multiplexed identification, quantification and genotyping of infectious agents using a semiconductor biochip.Nature biotechnology · 2018Article
- Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in breast cancer.Nature communications · 2017Article
- Massively parallel nanowell-based single-cell gene expression profiling.BMC genomics · 2017Article
- Targeted resequencing and variant validation using pxlence PCR assays.Biomolecular detection and quantification · 2016Article
- Progress and prospects of next-generation sequencing testing for inherited retinal dystrophy.Expert review of molecular diagnostics · 2015Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNext generation targeted resequencing is replacing Sanger sequencing at high pace in routine genetic diagnosis. The need for well validated, high quality enrichment platforms to complement the bench-top next generation sequencing devices is high.
resultsWe used the WaferGen Smartchip platform to perform highly parallelized PCR based target enrichment for a set of known cancer genes in a well characterized set of cancer cell lines from the NCI60 panel. Optimization of PCR assay design and cycling conditions resulted in a high enrichment efficiency. We provide proof of a high mutation rediscovery rate and have included technical replicates to enable SNP calling validation demonstrating the high reproducibility of our enrichment platform.
conclusionsHere we present our custom developed quantitative PCR based target enrichment platform. Using highly parallel nanoliter singleplex PCR reactions makes this a flexible and efficient platform. The high mutation validation rate shows this platform's promise as a targeted resequencing method for multi-gene routine sequencing diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.