ArticleClinical and experimental immunology2014
Nitric oxide production by polymorphonuclear leucocytes in infected cystic fibrosis sputum consumes oxygen.
Article in Clinical and experimental immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Microbiome and Inflammatory Biomarkers Associated With Palatal Wound Healing.Journal of periodontal research · 2025Trial
- NirA is a Cyanide-Tolerant Nitrite Reductase Which Protects Pseudomonas aeruginosa From Self-Poisoning.Environmental microbiology reports · 2025Article
- Ecological and evolutionary mechanisms driving within-patient emergence of antimicrobial resistance.Nature reviews. Microbiology · 2024Review
- Widespread detoxifying NO reductases impart a distinct isotopic fingerprint on NProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Inoculum Concentration Influences Pseudomonas aeruginosa Phenotype and Biofilm Architecture.Microbiology spectrum · 2022Article
- The structure-function relationship of Pseudomonas aeruginosa in infections and its influence on the microenvironment.FEMS microbiology reviews · 2022Review
- Increased sputum lactate during oral glucose tolerance test in cystic fibrosis.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Article
- Nitric-oxide-driven oxygen release in anoxiciScience · 2021Article
- Metabolic reprogramming in chondrocytes to promote mitochondrial respiration reduces downstream features of osteoarthritis.Scientific reports · 2021Article
- NO donors and NO delivery methods for controlling biofilms in chronic lung infections.Applied microbiology and biotechnology · 2021Review
- Immune Responses toFrontiers in immunology · 2021Review
- Corneal Infection Models: Tools to Investigate the Role of Biofilms in Bacterial Keratitis.Cells · 2020Review
- The OThe Journal of biological chemistry · 2020Article
- Improving antibiotic treatment of bacterial biofilm by hyperbaric oxygen therapy: Not just hot air.Biofilm · 2019Review
- Conceptual Model of Biofilm Antibiotic Tolerance That Integrates Phenomena of Diffusion, Metabolism, Gene Expression, and Physiology.Journal of bacteriology · 2019Article
- Modelling of ciprofloxacin killing enhanced by hyperbaric oxygen treatment in Pseudomonas aeruginosa PAO1 biofilms.PloS one · 2018Article
- Hyperbaric Oxygen Sensitizes Anoxic Pseudomonas aeruginosa Biofilm to Ciprofloxacin.Antimicrobial agents and chemotherapy · 2017Article
- Whether a novel drug delivery system can overcome the problem of biofilms in respiratory diseases?Drug delivery and translational research · 2017Review
- Inflammation: A Double-Edged Sword in the Response to Pseudomonas aeruginosa Infection.Journal of innate immunity · 2017Review
- A tale of two sites: how inflammation can reshape the microbiomes of the gut and lungs.Journal of leukocyte biology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chronic Pseudomonas aeruginosa lung infection in cystic fibrosis (CF) patients is characterized by persisting mucoid biofilms in hypoxic endobronchial mucus. These biofilms are surrounded by numerous polymorphonuclear leucocytes (PMNs), which consume a major part of present molecular oxygen (O(2)) due to production of superoxide (O(2)(-)). In this study, we show that the PMNs also consume O(2) for production of nitric oxide (NO) by the nitric oxide synthases (NOS) in the infected endobronchial mucus. Fresh expectorated sputum samples (n = 28) from chronically infected CF patients (n = 22) were analysed by quantifying and visualizing the NO production. NO production was detected by optode measurements combined with fluorescence microscopy, flow cytometry and spectrophotometry. Inhibition of nitric oxide synthases (NOS) with N(G) -monomethyl-L-arginine (L-NMMA) resulted in reduced O(2) consumption (P < 0·0008, n = 8) and a lower fraction of cells with fluorescence from the NO-indicator 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM) (P < 0·002, n = 8). PMNs stained with DAF-FM and the superoxide indicator hydroethidine (HE) and host cells with inducible NOS (iNOS) were identified in the sputum. In addition, the production of the stable end-products of NO in CF sputum was correlated with the concentration of PMNs; NO(3)(-) (P < 0·04, r = 0·66, n = 10) and NO(2)(-) (P< 0·006, r = 0·78, n = 11). The present study suggests that besides consumption of O(2) for production of reactive oxygen species, the PMNs in CF sputum also consume O(2) for production of NO.
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Registered trials
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