ArticleJournal of electroanalytical chemistry (Lausanne, Switzerland)2014
Square-wave voltammetry assays for glycoproteins on nanoporous gold.
Article in Journal of electroanalytical chemistry (Lausanne, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Facile Fabrication of Hierarchical Multimodal Nanoporous Gold (hm-NPG) via a Polysaccharide Polymer Template Method.Nanomaterials (Basel, Switzerland) · 2026Article
- Research progress on the synthesis process, detection method, pharmacological effects and application of glycocholic acid.Frontiers in pharmacology · 2024Review
- Facile fabrication of hierarchically nanostructured gold electrode for bio-electrochemical applications.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2022Article
- Electrochemical Discrimination of Salbutamol from Its Excipients in VentolinSensors (Basel, Switzerland) · 2021Article
- Nanoporous Gold Monolith for High Loading of Unmodified Doxorubicin and Sustained Co-Release of Doxorubicin-Rapamycin.Nanomaterials (Basel, Switzerland) · 2021Article
- A pH sensitive thiolated β-cyclodextrin-modified nanoporous gold for controlled release of doxorubicin.Journal of drug delivery science and technology · 2020Article
- Recent Advances in the Application of Glycan-Modified Self-Assembled Monolayers.Advances in chemistry research · 2020Article
- Preparation, Modification, Characterization, and Biosensing Application of Nanoporous Gold Using Electrochemical Techniques.Nanomaterials (Basel, Switzerland) · 2018Review
- Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes.Medicinal research reviews · 2017Review
- Enzyme Immobilization on Nanoporous Gold: A Review.Biochemistry insights · 2017Review
- Electrochemical impedance spectroscopy study of carbohydrate-terminated alkanethiol monolayers on nanoporous gold: Implications for pore wetting.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2016Article
- Electrochemical Impedance Spectroscopy Study of Concanavalin A Binding to Self-Assembled Monolayers of Mannosides on Gold Wire Electrodes.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2016Article
- Recent Progress in Lectin-Based Biosensors.Materials (Basel, Switzerland) · 2015Review
- Electrochemical synthesis of nanostructured gold film for the study of carbohydrate-lectin interactions using localized surface plasmon resonance spectroscopy.Carbohydrate research · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Electrochemical enzyme-linked lectinsorbent assays (ELLA) were developed using nanoporous gold (NPG) as a solid support for protein immobilization and as an electrode for the electrochemical determination of the product of the reaction between alkaline phosphatase (ALP) and p-aminophenyl phosphate (p-APP), which is p-aminophenol (p-AP). Glycoproteins or concanavalin A (Con A) and ALP conjugates were covalently immobilized onto lipoic acid self-assembled monolayers on NPG. The binding of Con A - ALP (or soybean agglutinin - ALP) conjugate to glycoproteins covalently immobilized on NPG and subsequent incubation with p-APP substrate was found to result in square-wave voltammograms whose peak difference current varied with the identity of the glycoprotein. NPG presenting covalently bound glycoproteins was used as the basis for a competitive electrochemical assay for glycoproteins in solution (transferrin and IgG). A kinetic ELLA based on steric hindrance of the enzyme-substrate reaction and hence reduced enzymatic reaction rate after glycoprotein binding is demonstrated using immobilized Con A-ALP conjugates. Using the immobilized Con A-ALP conjugate, the binding affinity of immunoglobulin G (IgG) was found to be 105 nM, and that for transferrin was found to be 650 nM. Minimal interference was observed in the presence of 5 mg mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.