ArticleApplied and environmental microbiology2014
Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers, 1 of them a synthesis that pooled it.
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Who cites it
107 citing papers in PubMed, 1 synthesis or guideline pooled it, 294 citations in OpenAlex.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Characterizing fecal bioaerosols in dairy barns using microbial source tracking markers and bovine enteric viruses.Microbiology spectrum · 2026Article
- Novel Bacteroides bacteriophage-like quantitative real-time PCR assays for human sewage pollution measurement.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Associations between Fecal Contamination of the Household Environment and Enteric Pathogen Detection in Children Living in Maputo, Mozambique.Environment & health (Washington, D.C.) · 2025Article
- Impact of sanitation system types on residential and environmental presence of human waste and parasites in Alabama.Infectious diseases of poverty · 2025Article
- Aircraft lavatory wastewater surveillance for movement of antimicrobial resistance genes: a proof-of-concept study.Microbiology spectrum · 2025Article
- Comparative evaluation of heating instruments for detectingJournal of water and health · 2025Article
- Wastewater surveillance for Salmonella Typhi and its association with seroincidence of enteric fever in Vellore, India.PLoS neglected tropical diseases · 2025Article
- The probability of detecting host-specific microbial source tracking markers in surface waters was strongly associated with method and season.Microbiology spectrum · 2025Article
- Evaluating Nanotrap Microbiome Particles as A Wastewater Viral Concentration Method.Food and environmental virology · 2025Article
- Temporal changes in fecal indicator bacteria and potential waterborne pathogens at Sequiota Spring: from sewer to spring.Frontiers in microbiology · 2025Article
- Enteric Pathogens in Humans, Domesticated Animals, and Drinking Water in a Low-Income Urban Area of Nairobi, Kenya.Environmental science & technology · 2024Article
- Performance of Conditional Random Forest and Regression Models at Predicting Human Fecal Contamination of Produce Irrigation Ponds in the Southeastern United States.ACS ES&T water · 2024Article
- Quantitative fecal pollution assessment with bacterial, viral, and molecular methods in small stream tributaries.The Science of the total environment · 2024Article
- Campylobacteriosis Outbreak Linked to Municipal Water, Nebraska, USA, 2021Emerging infectious diseases · 2024Review
- Advancing Source Tracking: Systematic Review and Source-Specific Genome Database Curation of Fecally Shed Prokaryotes.Environmental science & technology letters · 2024Article
- Distribution of host-specific Bacteriodales marker genes in water sources of selected rural areas of Vhembe District, South Africa.Scientific reports · 2024Article
- Quantitative fecal source characterization of urban municipal storm sewer system outfall 'wet' and 'dry' weather discharges.Water research · 2024Article
- Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.The Science of the total environment · 2024Article
- Assessing the nucleic acid decay of human wastewater markers and enteric viruses in estuarine waters in Sydney, Australia.The Science of the total environment · 2024Article
47 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 6 institutions in 1 country.
Funding
Abstract
Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.