ArticleThe Journal of biological chemistry2014
Degradation of phycobilisomes in Synechocystis sp. PCC6803: evidence for essential formation of an NblA1/NblA2 heterodimer and its codegradation by A Clp protease complex.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Comparative study of phycoerythrobilin synthases for fine-tuning photosynthetic light-harvesting complexes, phycobilisomes.Scientific reports · 2026Article
- How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress: The Role of Small Proteins in Cyanobacterial Stress Responses.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803 substrains.FEBS open bio · 2023Article
- Article
- Genetic Responses of Metabolically ActiveMicroorganisms · 2021Article
- The role of rhizofiltration and allelopathy on the removal of cyanobacteria in a continuous flow system.Environmental science and pollution research international · 2021Article
- Article
- Discovery of a small protein factor involved in the coordinated degradation of phycobilisomes in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phycobilisome breakdown effector NblD is required to maintain the cellular amino acid composition during nitrogen starvation.Journal of bacteriology · 2021Article
- Assessment of Protein Content and Phosphorylation Level inMolecules (Basel, Switzerland) · 2020Article
- Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing CyanobacteriumBiomolecules · 2019Article
- Global Transcriptional Profiling of the CyanobacteriumFrontiers in microbiology · 2019Article
- Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening.Molecular & cellular proteomics : MCP · 2018Article
- Adaptive laboratory evolution of cadmium tolerance inBiotechnology for biofuels · 2018Article
- NsrR1, a Nitrogen Stress-Repressed sRNA, Contributes to the Regulation ofFrontiers in microbiology · 2018Article
- Structures and enzymatic mechanisms of phycobiliprotein lyases CpcE/F and PecE/F.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Population-level coordination of pigment response in individual cyanobacterial cells under altered nitrogen levels.Photosynthesis research · 2017Article
- The proteolysis adaptor, NblA, binds to the N-terminus of β-phycocyanin: Implications for the mechanism of phycobilisome degradation.Photosynthesis research · 2017Article
- Phycobilisome truncation causes widespread proteome changes in Synechocystis sp. PCC 6803.PloS one · 2017Article
- β-Carotene influences the phycobilisome antenna of cyanobacterium Synechocystis sp. PCC 6803.Photosynthesis research · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When cyanobacteria acclimate to nitrogen deficiency, they degrade their large (3-5-MDa), light-harvesting complexes, the phycobilisomes. This massive, yet specific, intracellular degradation of the pigmented phycobiliproteins causes a color change of cyanobacterial cultures from blue-green to yellow-green, a process referred to as chlorosis or bleaching. Phycobilisome degradation is induced by expression of the nblA gene, which encodes a protein of ~7 kDa. NblA most likely acts as an adaptor protein that guides a Clp protease to the phycobiliproteins, thereby initiating the degradation process. Most cyanobacteria and red algae possess just one nblA-homologous gene. As an exception, the widely used "model organism" Synechocystis sp. PCC6803 expresses two such genes, nblA16803 and nblA26803, both of whose products are required for phycobilisome degradation. Here, we demonstrate that the two NblA proteins heterodimerize in vitro and in vivo using pull-down assays and a Förster energy-transfer approach, respectively. We further show that the NblA proteins form a ternary complex with ClpC (the HSP100 chaperone partner of Clp proteases) and phycobiliproteins in vitro. This complex is susceptible to ATP-dependent degradation by a Clp protease, a finding that supports a proposed mechanism of the degradation process. Expression of the single nblA gene encoded by the genome of the N2-fixing, filamentous cyanobacterium Nostoc sp. PCC7120 in the nblA1/nblA2 mutant of Synechocystis sp. PCC6803 induced phycobilisome degradation, suggesting that the function of the NblA heterodimer of Synechocystis sp. PCC6803 is combined in the homodimeric protein of Nostoc sp. PCC7120.
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