ArticleThe Plant cell2014
Ca2+-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers, 1 of them a synthesis that pooled it.
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Who cites it
136 citing papers in PubMed, 1 synthesis or guideline pooled it, 304 citations in OpenAlex.
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Reproductive thermotolerance: from flower development to seed initiation.The New phytologist · 2026Review
- Reactive Oxygen Species in Crop Plants: Production, Detoxification, Signaling, and Molecular Cross-Talk.Plants (Basel, Switzerland) · 2026Review
- Reactive oxygen species in plants: spatiotemporal organization, redox signaling, and stress adaptation.Planta · 2026Review
- PbrFEN-1 positively regulates pear pollen tube growth and is involved in the self-incompatibility response.Plant reproduction · 2026Article
- A simplePlant biotechnology (Tokyo, Japan) · 2026Article
- Papaver S-determinants trigger mitochondrially derived ROS production and disrupt energy metabolism in incompatible pollen tubes.The Plant cell · 2026Article
- The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast.Plant biology (Stuttgart, Germany) · 2026Article
- Endophytic extract Zhinengcong alleviates salt stress-induced reproductive defects in Solanum lycopersicum.BMC plant biology · 2026Article
- MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa.Plant biotechnology journal · 2026Article
- Premature pollen development hinders autonomous self-pollination and promotes insect pollination in soybean (Glycine max L.).Scientific reports · 2026Article
- Reactive Oxygen Species Homeostasis Regulates Pistil Development and Pollination inPlants (Basel, Switzerland) · 2026Article
- Physiological and transcriptomic insights into the molecular mechanisms of salt stress adaptation in Gardenia jasminoides.BMC plant biology · 2026Article
- PbrRALF5/10 prevents incompatible pollen tube death by reconstructing the methyl-esterified pectin and reactive oxygen species metabolism of pear in vitro.Plant molecular biology · 2025Article
- Reactive oxygen species act as signaling molecules to control root hair initiation and tip growth.The New phytologist · 2025Review
- Antioxidant Defense Systems in Plants: Mechanisms, Regulation, and Biotechnological Strategies for Enhanced Oxidative Stress Tolerance.Life (Basel, Switzerland) · 2025Review
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
- Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato.The Plant cell · 2025Article
- CTB6 Confers Cold Tolerance at the Booting Stage by Maintaining Tapetum Development in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
76 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In flowering plants, pollen germinates on the stigma and pollen tubes grow through the style to fertilize the ovules. Enzymatic production of reactive oxygen species (ROS) has been suggested to be involved in pollen tube tip growth. Here, we characterized the function and regulation of the NADPH oxidases RbohH and RbohJ (Respiratory burst oxidase homolog H and J) in pollen tubes in Arabidopsis thaliana. In the rbohH and rbohJ single mutants, pollen tube tip growth was comparable to that of the wild type; however, tip growth was severely impaired in the double mutant. In vivo imaging showed that ROS accumulation in the pollen tube was impaired in the double mutant. Both RbohH and RbohJ, which contain Ca(2+) binding EF-hand motifs, possessed Ca(2+)-induced ROS-producing activity and localized at the plasma membrane of the pollen tube tip. Point mutations in the EF-hand motifs impaired Ca(2+)-induced ROS production and complementation of the double mutant phenotype. We also showed that a protein phosphatase inhibitor enhanced the Ca(2+)-induced ROS-producing activity of RbohH and RbohJ, suggesting their synergistic activation by protein phosphorylation and Ca(2+). Our results suggest that ROS production by RbohH and RbohJ is essential for proper pollen tube tip growth, and furthermore, that Ca(2+)-induced ROS positive feedback regulation is conserved in the polarized cell growth to shape the long tubular cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.