Evidence map›Paper›PMID 24610332›Full record

ArticleJournal of microbiology (Seoul, Korea)2014

Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test.

Hee-Jung Lee, Kyung-Il Min, Ki Hoon Park, Hyo Jung Choi, Min-Kyoung Kim, Chi-Young Ahn, Young-Jin Hong, Young Bong Kim

Open access · bronzeAbstract readValidation Study
In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Pseudotyped Virus for Flaviviridae.Advances in experimental medicine and biology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Hee-Jung LeeDepartment of Bio-industrial technologies, Konkuk University, Seoul, 143-701, Republic of Korea.
Kyung-Il Min
Ki Hoon Park
Hyo Jung Choi
Min-Kyoung Kim
Chi-Young Ahn
Young-Jin Hong
Young Bong Kim
Konkuk University · KRNational Institute of Food and Drug Safety Evaluation · KRInha University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV-PV). JEV-PV-based neutralization assay offers several advantages compared with the current standard plaque-reduction neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque reduction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.

Indexed as

Antigens, ViralAntibodies, NeutralizingEncephalitis Viruses, JapaneseNeutralization TestsReproducibility of ResultsSensitivity and SpecificityViral Plaque AssayAntibodies, NeutralizingAntigens, Viral

Identifiers

PMID24610332
PMCPMC7090846
OpenAlexW2034442918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.