ArticlePloS one2014
Dre - Cre sequential recombination provides new tools for retinal ganglion cell labeling and manipulation in mice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- A spinal substrate for modular control of natural behavior.bioRxiv : the preprint server for biology · 2026Article
- Fate-Mapping Macrophages: From Ontogeny to Functions.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Review
- Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons.PloS one · 2023Article
- Construction and application of a multifunctional CHO cell platform utilizing Cre/Frontiers in bioengineering and biotechnology · 2023Article
- Article
- In Vivo Methods to Monitor Cardiomyocyte Proliferation.Journal of cardiovascular development and disease · 2022Review
- Identification of retinal ganglion cell types and brain nuclei expressing the transcription factor Brn3c/Pou4f3 using a Cre recombinase knock-in allele.The Journal of comparative neurology · 2021Article
- Article
- A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression.International journal of molecular sciences · 2019Article
- A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.Scientific reports · 2018Article
- The genome sequence of Escherichia coli tailed phage D6 and the diversity of Enterobacteriales circular plasmid prophages.Virology · 2018Article
- Molecular codes for cell type specification in Brn3 retinal ganglion cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transgenesis for pig models.Journal of veterinary science · 2016Review
- A knock-in allele of En1 expressing dre recombinase.Genesis (New York, N.Y. : 2000) · 2016Article
- Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers.Molecular vision · 2016Article
- Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies.G3 (Bethesda, Md.) · 2015Article
- Molecular, anatomical and functional changes in the retinal ganglion cells after optic nerve crush in mice.Documenta ophthalmologica. Advances in ophthalmology · 2015Article
- Article
- Strategies for site-specific recombination with high efficiency and precise spatiotemporal resolution.The Journal of biological chemistryReview
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundGenetic targeting methods have greatly advanced our understanding of many of the 20 Retinal Ganglion Cell (RGC) types conveying visual information from the eyes to the brain. However, the complexity and partial overlap of gene expression patterns in RGCs call for genetic intersectional or sparse labeling strategies. Loci carrying the Cre recombinase in conjunction with conditional knock-out, reporter or other genetic tools can be used for targeted cell type ablation and functional manipulation of specific cell populations. The three members of the Pou4f family of transcription factors, Brn3a, Brn3b and Brn3c, expressed early during RGC development and in combinatorial pattern amongst RGC types are excellent candidates for such gene manipulations. METHODS AND
findingsWe generated conditional Cre knock-in alleles at the Brn3a and Brn3b loci, Brn3a(CKOCre) and Brn3b(CKOCre). When crossed to mice expressing the Dre recombinase, the endogenous Brn3 gene expressed by Brn3a(CKOCre) or Brn3b(CKOCre) is removed and replaced with a Cre recombinase, generating Brn3a(Cre) and Brn3b(Cre) knock-in alleles. Surprisingly both Brn3a(Cre) and Brn3b(Cre) knock-in alleles induce early ubiquitous recombination, consistent with germline expression. However in later stages of development, their expression is limited to the expected endogenous pattern of the Brn3a and Brn3b genes. We use the Brn3a(Cre) and Brn3b(Cre) alleles to target a Cre dependent Adeno Associated Virus (AAV) reporter to RGCs and demonstrate its use in morphological characterization, early postnatal gene delivery and tracing the expression of Brn3 genes in RGCs.
conclusionsDre recombinase effectively recombines the Brn3a(CKOCre) and Brn3b(CKOCre) alleles containing its roxP target sites. Sequential Dre to Cre recombination reveals Brn3a and Brn3b expression in early mouse development. The generated Brn3a(Cre) and Brn3b(Cre) alleles are useful tools that can target exogenously delivered Cre dependent reagents to RGCs in early postnatal development, opening up a large range of potential applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.