ArticlePloS one2014
Deep RNA sequencing reveals hidden features and dynamics of early gene transcription in Paramecium bursaria chlorella virus 1.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Infection cycles of viruses of the phylum Nucleocytoviricota.Nature reviews. Microbiology · 2026Review
- Diversity and taxonomy of giant algal viruses: chloroviruses lead the way.Journal of virology · 2026Review
- Adenine DNA methylation associated with transcriptionally permissive chromatin is widespread across eukaryotes.Nature genetics · 2025Article
- Ultrastructural and transcriptional changes during a giant virus infection of a green alga.Npj viruses · 2025Article
- Genomics and evolutionary analysis ofJournal of virology · 2024Article
- Early-Phase Drive to the Precursor Pool: Chloroviruses Dive into the Deep End of Nucleotide Metabolism.Viruses · 2023Article
- Review
- Functional Genomic Analyses Reveal an Open Pan-genome for the Chloroviruses and a Potential for Genetic Innovation in New Isolates.Journal of virology · 2022Article
- Strong selection and high mutation supply characterize experimental Chlorovirus evolution.Virus evolution · 2022Article
- RNA Sequencing of Medusavirus Suggests Remodeling of the Host Nuclear Environment at an Early Infection Stage.Microbiology spectrum · 2021Article
- Comparative Analysis of Transcriptional Regulation Patterns: Understanding the Gene Expression Profile inPathogens (Basel, Switzerland) · 2021Review
- Cloning and Characterization of a New Chitosanase From a Deep-Sea BacteriumFrontiers in microbiology · 2021Article
- Metatranscriptome Library Preparation Influences Analyses of Viral Community Activity During a Brown Tide Bloom.Frontiers in microbiology · 2021Article
- Diversity of tRNA Clusters in the Chloroviruses.Viruses · 2020Article
- A single-cell view on alga-virus interactions reveals sequential transcriptional programs and infection states.Science advances · 2020Article
- Structural and Proteomic Studies of theFrontiers in microbiology · 2020Article
- Analysis of a Marseillevirus Transcriptome Reveals Temporal Gene Expression Profile and Host Transcriptional Shift.Frontiers in microbiology · 2020Article
- SMRT Sequencing of Paramecium Bursaria Chlorella Virus-1 Reveals Diverse Methylation Stability in Adenines Targeted by Restriction Modification Systems.Frontiers in microbiology · 2020Article
- Chloroviruses.Viruses · 2019Review
- Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host.PloS one · 2019Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of the genus Chlorovirus (family Phycodnaviridae) that infects the unicellular, eukaryotic green alga Chlorella variabilis NC64A. The 331-kb PBCV-1 genome contains 416 major open reading frames. A mRNA-seq approach was used to analyze PBCV-1 transcriptomes at 6 progressive times during the first hour of infection. The alignment of 17 million reads to the PBCV-1 genome allowed the construction of single-base transcriptome maps. Significant transcription was detected for a subset of 50 viral genes as soon as 7 min after infection. By 20 min post infection (p.i.), transcripts were detected for most PBCV-1 genes and transcript levels continued to increase globally up to 60 min p.i., at which time 41% or the poly (A+)-containing RNAs in the infected cells mapped to the PBCV-1 genome. For some viral genes, the number of transcripts in the latter time points (20 to 60 min p.i.) was much higher than that of the most highly expressed host genes. RNA-seq data revealed putative polyadenylation signal sequences in PBCV-1 genes that were identical to the polyadenylation signal AAUAAA of green algae. Several transcripts have an RNA fragment excised. However, the frequency of excision and the resulting putative shortened protein products suggest that most of these excision events have no functional role but are probably the result of the activity of misled splicesomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.