ArticleNature methods2014
Dynamic characterization of growth and gene expression using high-throughput automated flow cytometry.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Nestin in multiple myeloma: emerging insights into a potential therapeutic target.Frontiers in oncology · 2025Review
- Analysis and design of single-cell experiments to harvest fluctuation information while rejecting measurement noise.Frontiers in cell and developmental biology · 2023Article
- Enhancing bioreactor arrays for automated measurements and reactive control with ReacSight.Nature communications · 2022Article
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- High-Pressure Microfluidics for Ultra-Fast Microbial Phenotyping.Frontiers in microbiology · 2022Article
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- Immune co-culture cell microarray - a feasible tool for high-throughput functional investigation of lymphocyte-cancer interactions.Oncoimmunology · 2020Article
- Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation.Nature communications · 2018Article
- Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER.Nature biotechnology · 2018Article
- Article
- Reconstructing the regulatory circuit of cell fate determination in yeast mating response.PLoS computational biology · 2017Article
- Robust Synthetic Circuits for Two-Dimensional Control of Gene Expression in Yeast.ACS synthetic biology · 2017Article
- Article
- Label-free cell cycle analysis for high-throughput imaging flow cytometry.Nature communications · 2016Article
- Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation.Journal of cellular physiology · 2015Article
- Using dynamic noise propagation to infer causal regulatory relationships in biochemical networks.ACS synthetic biology · 2015Article
- Population diversification in a yeast metabolic program promotes anticipation of environmental shifts.PLoS biology · 2015Article
- Delayed Ras/PKA signaling augments the unfolded protein response.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The author file: Hana El-Samad.Nature methods · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cells adjust to changes in environmental conditions using complex regulatory programs. These cellular programs are the result of an intricate interplay between gene expression, cellular growth and protein degradation. Technologies that enable simultaneous and time-resolved measurements of these variables are necessary to dissect cellular homeostatic strategies. Here we report the development of an automated flow cytometry robotic setup that enables real-time measurement of precise and simultaneous relative growth and protein synthesis rates of multiplexed microbial populations across many conditions. These measurements generate quantitative profiles of dynamically evolving protein synthesis and degradation rates. We demonstrate this setup in the context of gene regulation of the unfolded protein response (UPR) of Saccharomyces cerevisiae and uncover a dynamic and complex landscape of gene expression, growth dynamics and proteolysis following perturbations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.