ArticleJournal of dentistry2014
Extracts of irradiated mature human tooth crowns contain MMP-20 protein and activity.
Article in Journal of dentistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- The impact of head and neck radiotherapy on the dentine-enamel junction: a systematic review.Medicina oral, patologia oral y cirugia bucal · 2020Pooled it
- Impact of radiotherapy on the morphological and compositional structure of intra-radicular dentin.Brazilian dental journalTrial
- Microshear bond strength of conventional and bioactive restorative materials to irradiated and non-irradiated dentin: an in vitro study.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2025Article
- Article
- Acid challenge exacerbates activation of matrix metalloproteinases in permanent teeth undergoing radiotherapy.Brazilian oral research · 2024Article
- Article
- Effects of radiation therapy on the dislocation resistance of root canal sealers applied to dentin and the sealer-dentin interface: a pilot study.Restorative dentistry & endodontics · 2021Article
- Effect of different solutions in reversing the damage caused by radiotherapy in dentin structure.Medicina oral, patologia oral y cirugia bucal · 2020Article
- Chemical analysis of in vivo-irradiated dentine of head and neck cancer patients by ATR-FTIR and Raman spectroscopy.Clinical oral investigations · 2019Article
- IMRT delivers lower radiation doses to dental structures than 3DRT in head and neck cancer patients.Radiation oncology (London, England) · 2016Article
- Radiotherapy effect on nano-mechanical properties and chemical composition of enamel and dentine.Archives of oral biology · 2015Article
- Type IV collagen is a novel DEJ biomarker that is reduced by radiotherapy.Journal of dental research · 2014Article
- Activation of gelatinases in permanent human teeth after different experimental radiotherapy protocols.Brazilian dental journalArticle
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesWe recently demonstrated a significant correlation between enamel delamination and tooth-level radiation dose in oral cancer patients. Since radiation can induce the synthesis and activation of matrix metalloproteinases, we hypothesized that irradiated teeth may contain active matrix metalloproteinases. MATERIALS AND
methodsExtracted teeth from oral cancer patients treated with radiotherapy and from healthy subjects were compared. Extracted mature third molars from healthy subjects were irradiated in vitro and/or incubated for 0-6 months at 37°C. All teeth were then pulverized, extracted, and extracts subjected to proteomic and enzymatic analyses.
resultsScreening of irradiated crown extracts using mass spectrometry identified MMP-20 (enamelysin) which is expressed developmentally in dentine and enamel but believed to be removed prior to tooth eruption. MMP-20 was composed of catalytically active forms at Mr=43, 41, 24 and 22kDa and was immunolocalized predominantly to the morphological dentine enamel junction. The proportion of different sized MMP-20 forms changed with incubation and irradiation. While the pattern was not altered directly by irradiation of healthy teeth with 70Gy, subsequent incubation at 37°C for 3-6 months with or without prior irradiation caused the proportion of Mr=24-22kDa MMP-20 bands to increase dramatically. Extracts of teeth from oral cancer patients who received >70Gy radiation also contained relatively more 24 and 22kDa MMP-20 than those of healthy age-related teeth.
conclusionMMP-20 is a radiation-resistant component of mature tooth crowns enriched in the dentine-enamel. We speculate that MMP-20 catalyzed degradation of organic matrix at this site could lead to enamel delamination associated with oral cancer radiotherapy.
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