ArticleJournal of structural biology2014
Cryo-EM analysis of the organization of BclA and BxpB in the Bacillus anthracis exosporium.
Article in Journal of structural biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Helical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain.Nature communications · 2024Article
- Article
- Emerging enzyme surface display systems for waste resource recovery.Environmental microbiology · 2023Review
- Roles and Organization of BxpB (ExsFA) and ExsFB in the Exosporium Outer Basal Layer of Bacillus anthracis.Journal of bacteriology · 2022Article
- ExsY, CotY, and CotE Effects on Bacillus anthracis Outer Spore Layer Architecture.Journal of bacteriology · 2022Article
- Localization of the CotY and ExsY proteins to the exosporium basal layer of Bacillus anthracis.MicrobiologyOpen · 2022Article
- The Morphogenetic Protein CotE Positions Exosporium Proteins CotY and ExsY during Sporulation of Bacillus cereus.mSphere · 2021Article
- Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review).Sovremennye tekhnologii v meditsine · 2021Review
- When Appearance Misleads: The Role of the Entomopathogen Surface in the Relationship with Its Host.Insects · 2020Review
- Molecular tiling on the surface of a bacterial spore - the exosporium of the Bacillus anthracis/cereus/thuringiensis group.Molecular microbiology · 2017Article
- Assembly of the outermost spore layer: pieces of the puzzle are coming together.Molecular microbiology · 2017Article
- Surviving Between Hosts: Sporulation and Transmission.Microbiology spectrum · 2016Review
- Glycosylation of BclA Glycoprotein from Bacillus cereus and Bacillus anthracis Exosporium Is Domain-specific.The Journal of biological chemistry · 2016Article
- The Regulation of Exosporium-Related Genes in Bacillus thuringiensis.Scientific reports · 2016Article
- The Exosporium Layer of Bacterial Spores: a Connection to the Environment and the Infected Host.Microbiology and molecular biology reviews : MMBR · 2015Review
- Contribution of S-layer proteins to the mosquitocidal activity of Lysinibacillus sphaericus.PloS one · 2014Article
- Article
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Authors and funding
4 authors.
Funding
Abstract
Bacillus anthracis and other pathogenic Bacillus species form spores that are surrounded by an exosporium, a balloon-like layer that acts as the outer permeability barrier of the spore and contributes to spore survival and virulence. The exosporium consists of a hair-like nap and a paracrystalline basal layer. The filaments of the nap are comprised of trimers of the collagen-like glycoprotein BclA, while the basal layer contains approximately 20 different proteins. One of these proteins, BxpB, forms tight complexes with BclA and is required for attachment of essentially all BclA filaments to the basal layer. Another basal layer protein, ExsB, is required for the stable attachment of the exosporium to the spore. To determine the organization of BclA and BxpB within the exosporium, we used cryo-electron microscopy, cryo-sectioning and crystallographic analysis of negatively stained exosporium fragments to compare wildtype spores and mutant spores lacking BclA, BxpB or ExsB (ΔbclA, ΔbxpB and ΔexsB spores, respectively). The trimeric BclA filaments are attached to basal layer surface protrusions that appear to be trimers of BxpB. The protrusions interact with a crystalline layer of hexagonal subunits formed by other basal layer proteins. Although ΔbxpB spores retain the hexagonal subunits, the basal layer is not organized with crystalline order and lacks basal layer protrusions and most BclA filaments, indicating a central role for BxpB in exosporium organization.
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