ArticlePlant methods2014
Phenotypic analysis of the Arabidopsis heat stress response during germination and early seedling development.
Article in Plant methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Root and microbiome synergy in plant heat stress resilience: epigenetic regulation as a frontier for future research.Journal of experimental botany · 2026Review
- Changes in both gene expression and intron retention tune themicroPublication biology · 2026Article
- The N-degron pathway governs autophagy to promote thermotolerance in Arabidopsis.Nature communications · 2025Article
- Wheat COBRA-like GeneInternational journal of molecular sciences · 2025Article
- ABC transporter activity is affected by the size of lipid nanodiscs.FEBS letters · 2025Article
- CINV1 and CINV2 are required for increased tolerance to diverse stresses after ethylene-pretreatment of germinating seeds.PloS one · 2025Article
- Exploring the Relationship Between Gene Expression and Low-Frequency Somatic Mutations in Arabidopsis with Duplex Sequencing.Genome biology and evolution · 2024Article
- Bet-hedging and variability in plant development: seed germination and beyond.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Investigating low frequency somatic mutations inbioRxiv : the preprint server for biology · 2024Article
- Demographic consequences of an extreme heat wave are mitigated by spatial heterogeneity in an annual monkeyflower.Ecology and evolution · 2023Article
- Ethylene-mediated metabolic priming increases photosynthesis and metabolism to enhance plant growth and stress tolerance.PNAS nexus · 2023Article
- A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance.Plant methods · 2023Article
- Physiological responses induced by phospholipase C isoform 5 upon heat stress inFrontiers in plant science · 2023Article
- Heat Stress Resistance Mechanisms of Two Cucumber Varieties from Different Regions.International journal of molecular sciences · 2022Article
- Thermo-Priming Mediated Cellular Networks for Abiotic Stress Management in Plants.Frontiers in plant science · 2022Review
- Article
- The Epigenetic Mechanisms Underlying Thermomorphogenesis and Heat Stress Responses inPlants (Basel, Switzerland) · 2021Review
- Expression profiling of H3K27me3 demethylase genes during plant development and in response to environmental stress inPlant signaling & behavior · 2021Article
- Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells.Nature communications · 2021Article
- H3K27me3 demethylases alter HSP22 and HSP17.6C expression in response to recurring heat in Arabidopsis.Nature communications · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhenotypic characterization of Arabidopsis thaliana gain- and loss-of-function mutants is a delicate and meticulous task that often involves the analysis of multiple parameters. Arabidopsis heat tolerance has been evaluated based on direct assessments that include seed germination, seedling survival, hypocotyl and root elongation, or indirect measurements such as chlorophyll content or ion leakage.
resultsIn an attempt to simplify the detection of heat stress-associated phenotypes, a collection of protocols for analysis of seed germination and seedling survival to heat treatment is proposed. Temperatures and lengths of heat treatments were combined into several heat tolerance assays, to be used as a primary approach for the search and characterization of basal and acquired heat tolerance-associated phenotypes at early developmental stages. The usefulness of this methodology was illustrated through the characterization of heat-related phenotypes in different Arabidopsis ecotypes as well as in gain- and loss-of-function mutants.
conclusionsThe use of standardized experimental protocols designed to detect temperature-related phenotypes is proposed. The suggested plate-based assays provide an appropriate framework of experimental conditions for detection of variability amongst natural accessions or mutants lines. Functional studies could be facilitated by using this inexpensive and undemanding approach.
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