ArticleBiotechnology for biofuels2014
Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Triacylglycerols at the crossroads of lipid remodeling and stress tolerance in plants.Plant cell reports · 2026Review
- PHR1-like 7 and phosphatidic acid oppositely regulate TAG degradation and seed oil accumulation in Arabidopsis.Plant communications · 2026Article
- Targets and strategies to design soybean seed composition traits.The plant genome · 2025Review
- Deciphering the role of monoacylglycerol lipases (MAGL) under abiotic stress and lipid metabolism in soybean (Glycine max L.).Plant biotechnology journal · 2025Article
- Article
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- Towards rational control of seed oil composition: dissecting cellular organization and flux control of lipid metabolism.Plant physiology · 2025Review
- Novel candidate genes and genetic basis analysis of kernel starch content in tropical maize.BMC plant biology · 2025Article
- Lipid droplets in plants: turnover and stress responses.Frontiers in plant science · 2025Review
- Genome-wide identification and mining elite allele variation of the Monoacylglycerol lipase (MAGL) gene family in upland cotton (Gossypium hirsutum L.).BMC plant biology · 2024Article
- Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement.Plant biotechnology journal · 2023Article
- Rice lipases: a conundrum in rice bran stabilization: a review on their impact and biotechnological interventions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Pyruvate transporter BnaBASS2 impacts seed oil accumulation in Brassica napus.Plant biotechnology journal · 2022Article
- Biogenesis and Lipase-Mediated Mobilization of Lipid Droplets in Plants.Plants (Basel, Switzerland) · 2022Review
- Suppression ofFrontiers in plant science · 2022Article
- Suppression ofFrontiers in plant science · 2022Article
- Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis.Frontiers in plant science · 2022Review
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTriacylglycerols (TAGs) are the most abundant form of storage oil in plants. They consist of three fatty acid chains (usually C16 or C18) covalently linked to glycerol. SDP1 is a specific lipase for the first step of TAG catabolism in Arabidopsis seeds. Arabidopsis mutants deficient in SDP1 accumulate high levels of oils, probably due to blockage in TAG degradation. We applied this knowledge from the model plant, Arabidopsis thaliana, to engineer increased seed oil content in the biodiesel plant Jatropha curcas using RNA interference (RNAi) technology.
resultsAs Jatropha is a biodiesel crop, any significant increase in its seed oil content would be an important agronomic trait. Using A. thaliana as a model plant, we found that a deficiency of SDP1 led to higher TAG accumulation and a larger number of oil bodies in seeds compared with wild type (Columbia-0; Col-0). We cloned Jatropha JcSDP1, and verified its function by complementation of the Arabidopsis sdp1-5 mutant. Taking advantage of the observation with Arabidopsis, we used RNAi technology to generate JcSDP1 deficiency in transgenic Jatropha. We found that Jatropha JcSDP1-RNAi plants accumulated 13 to 30% higher total seed storage lipid, along with a 7% compensatory decrease in protein content, compared with control (CK; 35S:GFP) plants. Free fatty acid (FFA) content in seeds was reduced from 27% in control plants to 8.5% in JcSDP1-RNAi plants.
conclusionHere, we showed that SDP1 deficiency enhances seed oil accumulation in Arabidopsis. Based on this result, we generated SDP1-deficient transgenic Jatropha plants using by RNAi technology with a native JcSDP1 promoter to silence endogenous JcSDP1 expression. Seeds of Jatropha JcSDP1-RNAi plants accumulated up to 30% higher total lipid and had reduced FFA content compared with control (CK; 35S:GFP) plants. Our strategy of improving an important agronomic trait of Jatropha can be extended to other oil crops to yield higher seed oil.
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Registered trials
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