ArticleTissue engineering. Part A2014
Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- The Effect of Physical, Biochemical, and Electrical Culture Conditions on Articular Chondrocytes Used for Cartilage Tissue Engineering: A Narrative Review.International journal of molecular sciences · 2026Review
- Bioorthogonal Tuning of Hydrogel Stiffness Promotes Zonal Redifferentiation of Passaged Chondrocytes.bioRxiv : the preprint server for biology · 2026Article
- Tokay gecko tail regeneration involves temporally collinear expression of HOXC genes and early expression of satellite cell markers.BMC biology · 2025Article
- Article
- Targeting F-actin stress fibers to suppress the dedifferentiated phenotype in chondrocytes.European journal of cell biology · 2024Article
- Unlocking Potential: Low Bovine Serum Albumin Enhances the Chondrogenicity of Human Adipose-Derived Stromal Cells in Pellet Cultures.Biomolecules · 2024Article
- Three-Dimensional Spheroid Culture of Human Mesenchymal Stem Cells: Offering Therapeutic Advantages and In Vitro Glimpses of the In Vivo State.Stem cells translational medicine · 2023Review
- Article
- Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat.Food science of animal resources · 2022Review
- Strategies to Modulate the Redifferentiation of Chondrocytes.Frontiers in bioengineering and biotechnology · 2021Review
- Advanced protein adsorption properties of a novel silicate-based bioceramic: A proteomic analysis.Bioactive materials · 2021Article
- Collagen microsphere based 3D culture system for human osteoarthritis chondrocytes (hOACs).Scientific reports · 2019Article
- Impact of dexamethasone concentration on cartilage tissue formation from human synovial derived stem cells in vitro.Cytotechnology · 2018Article
- A high-glucose diet affects Achilles tendon healing in rats.Scientific reports · 2017Article
- Interplay between cytoskeletal polymerization and the chondrogenic phenotype in chondrocytes passaged in monolayer culture.Journal of anatomy · 2017Article
- An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.BioMed research international · 2017Article
- Efficient, Low-Cost Nucleofection of Passaged Chondrocytes.Cartilage · 2016Article
- Targeting the reorganization of F-actin for cell-based implantation cartilage repair therapies.Differentiation; research in biological diversityReview
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveOne of the factors preventing clinical application of regenerative medicine to degenerative cartilage diseases is a suitable source of cells. Chondrocytes, the only cell type of cartilage, grown in vitro under culture conditions to expand cell numbers lose their phenotype along with the ability to generate hyaline cartilaginous tissue. In this study we determine that a serum- and growth-factor-free three-dimensional (3D) culture system restores the ability of the passaged chondrocytes to form cartilage tissue in vitro, a process that involves sox9.
methodsBovine articular chondrocytes were passaged twice to allow for cell number expansion (P2) and cultured at high density on 3D collagen-type-II-coated membranes in high glucose content media supplemented with insulin and dexamethasone (SF3D). The cells were characterized after monolayer expansion and following 3D culture by flow cytometry, gene expression, and histology. The early changes in signaling transduction pathways during redifferentiation were characterized.
resultsThe P2 cells showed a progenitor-like antigen profile of 99% CD44(+) and 40% CD105(+) and a gene expression profile suggestive of interzone cells. P2 in SF3D expressed chondrogenic genes and accumulated extracellular matrix. Downregulating insulin receptor (IR) with HNMPA-(AM3) or the PI-3/AKT kinase pathway (activated by insulin treatment) with Wortmannin inhibited collagen synthesis. HNMPA-(AM3) reduced expression of Col2, Col11, and IR genes as well as Sox6 and -9. Co-immunoprecipitation and chromatin immunoprecipitation analyses of HNMPA-(AM3)-treated cells showed binding of the coactivators Sox6 and Med12 with Sox9 but reduced Sox9-Col2a1 binding.
conclusionsWe describe a novel culture method that allows for increase in the number of chondrocytes and promotes hyaline-like cartilage tissue formation in part by insulin-mediated Sox9-Col2a1 binding. The suitability of the tissue generated via this approach for use in joint repair needs to be examined in vivo.
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