ArticleMolecular endocrinology (Baltimore, Md.)2014
Genome-wide identification of AR-regulated genes translated in Sertoli cells in vivo using the RiboTag approach.
Article in Molecular endocrinology (Baltimore, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
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- Alpha4 Na,K-ATPase Localization and Expression Are Dynamic Aspects of Spermatogenesis and in Sperm Incubated Under Capacitating Conditions.International journal of molecular sciences · 2025Article
- Sertoli cells as a hub in testicular development and male reproductive.Frontiers in cell and developmental biology · 2025Review
- A comparative analysis of genes differentially expressed between rete testis cells and Sertoli cells of the mouse testis.Scientific reports · 2023Article
- Functions of Steroid Hormones in the Male Reproductive Tract as Revealed by Mouse Models.International journal of molecular sciences · 2023Review
- The Role of ZIP9 and Androgen Receptor in the Establishment of Tight Junctions between Adult Rat Sertoli Cells.Biology · 2022Article
- Concordant Androgen-Regulated Expression of Divergent Rhox5 Promoters in Sertoli Cells.Endocrinology · 2022Article
- Updates in Sertoli Cell-Mediated Signaling During Spermatogenesis and Advances in Restoring Sertoli Cell Function.Frontiers in endocrinology · 2022Review
- Revisiting the Characteristics of Testicular Germ Cell Lines GC-1(spg) and GC-2(spd)ts.Molecular biotechnology · 2021Article
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- Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS.iScience · 2021Article
- Androgen Actions in the Testis and the Regulation of Spermatogenesis.Advances in experimental medicine and biology · 2021Review
- Regulation of meiotic progression by Sertoli-cell androgen signaling.Molecular biology of the cell · 2020Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium.Frontiers in cellular neuroscience · 2020Article
- Entry into puberty is reflected in changes in hormone production but not in testicular receptor expression in Atlantic salmon (Salmo salar).Reproductive biology and endocrinology : RB&E · 2019Article
- A microRNA cluster in the Fragile-X region expressed during spermatogenesis targets FMR1.EMBO reports · 2019Article
- Global polysome analysis of normal and injured podocytes.American journal of physiology. Renal physiology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
An understanding of the molecular mechanisms by which androgens drive spermatogenesis has been thwarted by the fact that few consistent androgen receptor (AR) target genes have been identified. Here, we addressed this issue using next-generation sequencing coupled with the RiboTag approach, which purifies translated mRNAs expressed in cells that express cyclic recombinase (CRE). Using RiboTag mice expressing CRE in Sertoli cells (SCs), we identified genes expressed specifically in SCs in both prepubertal and adult mice. Unexpectedly, this analysis revealed that the SC-specific gene program is already largely defined at the initiation of spermatogenesis despite the subsequent dramatic maturational changes known to occur in SCs. To identify AR-regulated genes, we generated triple-mutant mice in which the SCs express the RiboTag but lack ARs. RNA sequencing analysis revealed hundreds of SC-expressed AR-regulated genes that had previously gone unnoticed, including suppressed genes involved in ovarian development. Comparison of the SC-enriched dataset with that from the whole testes allowed us to classify genes in terms of their degree of expression in SCs. This revealed that a greater fraction of AR-up-regulated genes than AR-down-regulated genes were expressed predominantly in SCs. Our results also revealed that AR signaling in SCs causes a large number of genes not detectably expressed in SCs to undergo altered expression, thereby providing genome-wide evidence for wide-scale communication between SCs and other cells. Taken together, our results identified novel classes of genes expressed in a hormone-dependent manner in different testicular cell subsets and highlight a new approach to analyze cell type-specific gene regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.