ArticleAnalytical chemistry2014
Simple and efficient method to purify DNA-protein conjugates and its sensing applications.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 53 citations in OpenAlex.
- Functionalizing Nucleic Acids: Synthesis and Purification Strategies for Bioconjugates as Biomaterials.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic Steps.Bio-protocol · 2025Article
- Article
- Micropreparative Gel Electrophoresis for Purification of Nanoscale Bioconjugates.Bioconjugate chemistry · 2024Article
- Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification.Mikrochimica acta · 2023Article
- Immunoglobulin E Detection Method Based on Cascade Enzymatic Reaction Utilizing Portable Personal Glucose Meter.Sensors (Basel, Switzerland) · 2021Article
- Ultrasensitive electrochemical detection of hepatitis C virus core antigen using terminal deoxynucleotidyl transferase amplification coupled with DNA nanowires.Mikrochimica acta · 2021Article
- Novel DNA-based T-Cell Activator Promotes Rapid T-Cell Activation and Expansion.Journal of immunotherapy (Hagerstown, Md. : 1997) · 2020Article
- A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.Chemical science · 2020Article
- Translating in vitro diagnostics from centralized laboratories to point-of-care locations using commercially-available handheld meters.Trends in analytical chemistry : TRAC · 2020Article
- Manipulating Enzymes Properties with DNA Nanostructures.Molecules (Basel, Switzerland) · 2019Review
- Rapid detection of cocaine using aptamer-based biosensor on an evanescent wave fibre platform.Royal Society open science · 2018Article
- An exploration of nucleic acid liquid biopsy using a glucose meter.Chemical science · 2018Article
- Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips.Angewandte Chemie (International ed. in English) · 2017Article
- A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers.Scientific reports · 2015Article
- Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health.Biotechnology advancesReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
DNA-protein conjugates are very useful in analytical chemistry for target recognition and signal amplification. While a number of methods for conjugating DNA with proteins are known, methods for purification of DNA-protein conjugates from reaction mixture containing unreacted proteins are much less investigated. In this work, a simple and efficient approach to purify DNA-invertase conjugates from reaction mixture via a biotin displacement strategy to release desthiobiotinylated DNA-invertase conjugates from streptavidin-coated magnetic beads was developed. The conjugates purified by this approach were utilized for quantitative detection of cocaine and DNA using a personal glucose meter through structure-switching DNA aptamer sensors and competitive DNA hybridization assays, respectively. In both cases, the purified DNA-invertase conjugates showed better performance compared to the same assays using unpurified conjugates. The approach demonstrated here can be further expanded to other DNA and proteins to generate purified DNA-protein conjugates for analytical and other applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.