ArticleViral immunology2014
Detection of influenza A/H1N1-specific human IgG-secreting B cells in older adults by ELISPOT assay.
Article in Viral immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Enterococcus durans YQ-6 Enhances Antioxidant Capacity and Immune Function in Leizhou Black Ducks.Probiotics and antimicrobial proteins · 2026Article
- Review
- Frequencies of SARS-CoV-2 Spike Protein-Specific Memory B Cells in Human PBMCs, Quantified by ELISPOT Assay.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Profiling the specificity of clonally expanded plasma cells during chronic viral infection by single-cell analysis.European journal of immunology · 2022Article
- Affinity Tag Coating Enables Reliable Detection of Antigen-Specific B Cells in Immunospot Assays.Cells · 2021Article
- Article
- Sex Differences in Older Adults' Immune Responses to Seasonal Influenza Vaccination.Frontiers in immunology · 2019Article
- Transcriptomic signatures of cellular and humoral immune responses in older adults after seasonal influenza vaccination identified by data-driven clustering.Scientific reports · 2018Article
- Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.Vaccine · 2016Article
- The composition of immune cells serves as a predictor of adaptive immunity in a cohort of 50- to 74-year-old adults.Immunology · 2016Article
- Vitamin D, leptin and impact on immune response to seasonal influenza A/H1N1 vaccine in older persons.Human vaccines & immunotherapeutics · 2016Article
- Immunosenescence-Related Transcriptomic and Immunologic Changes in Older Individuals Following Influenza Vaccination.Frontiers in immunology · 2016Article
- System-Wide Associations between DNA-Methylation, Gene Expression, and Humoral Immune Response to Influenza Vaccination.PloS one · 2016Article
- Article
- Profiles of influenza A/H1N1 vaccine response using hemagglutination-inhibition titers.Human vaccines & immunotherapeutics · 2015Article
- The impact of immunosenescence on humoral immune response variation after influenza A/H1N1 vaccination in older subjects.PloS one · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
B cells play an important role in humoral immunity and antibody production. Use of a B cell ELISPOT assay to quantify antigen-specific B cells can assist other assays to achieve a more complete profile of the humoral immune response after vaccination. We utilized a B cell ELISPOT assay to measure the number of influenza A/H1N1-specific B cells at key timepoints after seasonal influenza vaccination in 106 older adults (50-74 years of age). Blood was drawn from these subjects on Day 0, Day 3, Day 28, and Day 75 after vaccination to represent baseline, early, peak, and late response, respectively, of influenza A/H1N1-specific B cells. A significant increase in A/H1N1-specific B cells (median 36 spot-forming units/SFUs per 200,000 cells, p<0.0001) was seen on Day 28 compared to baseline and Day 3, and this number decreased (23 SFUs, p<0.0001) by Day 75, but not to baseline level. These data suggest that the B cell ELISPOT can be used to profile and monitor the humoral immune responses in older subjects after influenza vaccination, and serve as an immune signature marker.
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