ArticleFrontiers in cellular neuroscience2014
Six-color intravital two-photon imaging of brain tumors and their dynamic microenvironment.
Article in Frontiers in cellular neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 83 citations in OpenAlex.
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- CoSiDeX: A hyperspectral fluorescent protein resource for highly multiplexed imaging.bioRxiv : the preprint server for biology · 2025Article
- Electrochemical fluorescence modulation enables simultaneous multicolour imaging.Nature photonics · 2025Article
- Current trends in the characterization and monitoring of vascular response to cancer therapy.Cancer imaging : the official publication of the International Cancer Imaging Society · 2024Review
- Diving head-first into brain intravital microscopy.Frontiers in immunology · 2024Review
- Approaches to Improve EPR-Based Drug Delivery for Cancer Therapy and Diagnosis.Journal of personalized medicine · 2023Review
- SUFI: an automated approach to spectral unmixing of fluorescent multiplex images captured in mouse and post-mortem human brain tissues.BMC neuroscience · 2023Article
- Imaging mitochondria through bone in live mice using two-photon fluorescence microscopy with adaptive optics.Frontiers in neuroimaging · 2023Article
- Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range.International journal of molecular sciences · 2022Article
- Visualization of micro-agents and surroundings by real-time multicolor fluorescence microscopy.Scientific reports · 2022Article
- Multimodal imaging of the dynamic brain tumor microenvironment during glioblastoma progression and in response to treatment.iScience · 2022Article
- 3D imaging for driving cancer discovery.The EMBO journal · 2022Review
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- The complex interactions between the cellular and non-cellular components of the brain tumor microenvironmental landscape and their therapeutic implications.Frontiers in oncology · 2022Review
- Intravital Imaging of Hepatic Blood Biliary Barrier in Live Mice.Current protocols · 2021Article
- Single-laser-based simultaneous four-wavelength excitation source for femtosecond two-photon fluorescence microscopy.Biomedical optics express · 2021Article
- Improvement of the Similarity Spectral Unmixing Approach for Multiplexed Two-Photon Imaging by Linear Dimension Reduction of the Mixing Matrix.International journal of molecular sciences · 2021Article
- Label-Free Deep Profiling of the Tumor Microenvironment.Cancer research · 2021Article
- 3D Whole-Brain Imaging Approaches to Study Brain Tumors.Cancers · 2021Review
- Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of intravital studies on brain tumor in living animal so far rely on dual color imaging. We describe here a multiphoton imaging protocol to dynamically characterize the interactions between six cellular components in a living mouse. We applied this methodology to a clinically relevant glioblastoma multiforme (GBM) model designed in reporter mice with targeted cell populations labeled by fluorescent proteins of different colors. This model permitted us to make non-invasive longitudinal and multi-scale observations of cell-to-cell interactions. We provide examples of such 5D (x,y,z,t,color) images acquired on a daily basis from volumes of interest, covering most of the mouse parietal cortex at subcellular resolution. Spectral deconvolution allowed us to accurately separate each cell population as well as some components of the extracellular matrix. The technique represents a powerful tool for investigating how tumor progression is influenced by the interactions of tumor cells with host cells and the extracellular matrix micro-environment. It will be especially valuable for evaluating neuro-oncological drug efficacy and target specificity. The imaging protocol provided here can be easily translated to other mouse models of neuropathologies, and should also be of fundamental interest for investigations in other areas of systems biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.