ArticleChemistry (Weinheim an der Bergstrasse, Germany)2014
Isolation and characterization of precise dye/dendrimer ratios.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Distributions: The Importance of the Chemist's Molecular View for Biological Materials.Biomacromolecules · 2018Review
- Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.ACS biomaterials science & engineering · 2016Article
- Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios.Materials today (Kidlington, England) · 2015Article
- Quantitative Measurement of Cationic Polymer Vector and Polymer-pDNA Polyplex Intercalation into the Cell Plasma Membrane.ACS nano · 2015Article
- Fluorophore:dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime.Bioconjugate chemistry · 2015Article
- Poly(amidoamine) dendrimer-methotrexate conjugates: the mechanism of interaction with folate binding protein.Molecular pharmaceutics · 2014Article
- Multivalent polymers for drug delivery and imaging: the challenges of conjugation.Biomacromolecules · 2014Review
- Avidity mechanism of dendrimer-folic acid conjugates.Molecular pharmaceutics · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Fluorescent dyes are commonly conjugated to nanomaterials for imaging applications using stochastic synthesis conditions that result in a Poisson distribution of dye/particle ratios and therefore a broad range of photophysical and biodistribution properties. We report the isolation and characterization of generation 5 poly(amidoamine) (G5 PAMAM) dendrimer samples containing 1, 2, 3, and 4 fluorescein (FC) or 6-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dyes per polymer particle. For the fluorescein case, this was achieved by stochastically functionalizing dendrimer with a cyclooctyne "click" ligand, separation into sample containing precisely defined "click" ligand/particle ratios using reverse-phase high performance liquid chromatography (RP-HPLC), followed by reaction with excess azide-functionalized fluorescein dye. For the TAMRA samples, stochastically functionalized dendrimer was directly separated into precise dye/particle ratios using RP-HPLC. These materials were characterized using (1)H and (19)F NMR spectroscopy, RP-HPLC, UV/Vis and fluorescence spectroscopy, lifetime measurements, and MALDI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.