ArticleRadiation and environmental biophysics2014
Automated analysis of the cytokinesis-block micronucleus assay for radiation biodosimetry using imaging flow cytometry.
Article in Radiation and environmental biophysics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Application of an imaging flow cytometry γ-H2AX assay for biodosimetry using supervised machine learning.International journal of radiation biology · 2026Article
- Phytochemical Profile and In Vitro Cytotoxic, Genotoxic, and Antigenotoxic Evaluation ofInternational journal of molecular sciences · 2024Article
- Potentials of cytokinesis blocked micronucleus assay in radiation triage and biological dosimetry.Journal, genetic engineering & biotechnology · 2024Review
- A multi-biomarker micronucleus assay using imaging flow cytometry.Archives of toxicology · 2024Article
- Development of high-throughput systems for biodosimetry.Radiation protection dosimetry · 2023Article
- The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Imaging Flow Cytometry of Multi-Nuclearity.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Imaging flow cytometry: a primer.Nature reviews. Methods primers · 2022Article
- Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learning.Archives of toxicology · 2021Article
- The in vitro micronucleus assay using imaging flow cytometry and deep learning.NPJ systems biology and applications · 2021Article
- Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.Radiation research · 2019Article
- Automation of the in vitro micronucleus assay using the ImagestreamCytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Article
- The Application of Imaging Flow Cytometry to High-Throughput Biodosimetry.Genome integrity · 2017Review
- Liquid Handling Optimization in High-Throughput Biodosimetry Tool.Journal of medical devices · 2016Article
- Optimized automated data analysis for the cytokinesis-block micronucleus assay using imaging flow cytometry for high throughput radiation biodosimetry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Article
- Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay.Journal of radiation research · 2016Article
- EPRBioDose 2013: EPR applications and biological dosimetry.Radiation and environmental biophysics · 2014Article
- Multiparameter imaging flow cytometry-based cytokinesis-block micronucleus assay: Reduction of culture time and blood volume for improved efficiency.Mutation research. Genetic toxicology and environmental mutagenesisArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytokinesis-block micronucleus (CBMN) assay is employed in biological dosimetry to determine the dose of radiation to an exposed individual from the frequency of micronuclei (MN) in binucleated lymphocyte cells. The method has been partially automated for the use in mass casualty events, but it would be advantageous to further automate the method for increased throughput. Recently, automated image analysis has been successfully applied to the traditional, slide-scoring-based method of the CBMN assay. However, with the development of new technologies such as the imaging flow cytometer, it is now possible to adapt this microscope-based assay to an automated imaging flow cytometry method. The ImageStream(X) is an imaging flow cytometer that has adequate sensitivity to quantify radiation doses larger than 1 Gy while adding the increased throughput of traditional flow cytometry. The protocol and analysis presented in this work adapts the CBMN assay for the use on the ImageStream(X). Ex vivo-irradiated whole blood samples cultured for CBMN were analyzed on the ImageStream(X), and preliminary results indicate that binucleated cells and MN can be identified, imaged and enumerated automatically by imaging flow cytometry. Details of the method development, gating strategy and the dose response curve generated are presented and indicate that adaptation of the CBMN assay for the use with imaging flow cytometry has potential for high-throughput analysis following a mass casualty radiological event.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.