ArticleGenes & nutrition2014
Vitamin C supplementation modulates gene expression in peripheral blood mononuclear cells specifically upon an inflammatory stimulus: a pilot study in healthy subjects.
Article in Genes & nutrition, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Effect of six weeks 1000 mg/day vitamin C supplementation and healthy training in elderly women on genes expression associated with the immune response - a randomized controlled trial.Journal of the International Society of Sports Nutrition · 2021Trial
- Review
- The immune system as a guardian of health: micronutrient support and lifelong protection.Frontiers in nutrition · 2026Review
- Exploring the influence of vitamin C concentrations on the dynamics of RT-PCR assay reactions.Scientific reports · 2025Article
- Article
- Unlocking the potential of microRNAs: machine learning identifies key biomarkers for myocardial infarction diagnosis.Cardiovascular diabetology · 2023Article
- Ascorbate as a Bioactive Compound in Cancer Therapy: The Old Classic Strikes Back.Molecules (Basel, Switzerland) · 2022Review
- High-Dose Vitamin C Supplementation as a Legitimate Anti-SARS-CoV-2 Prophylaxis in Healthy Subjects-Yes or No?Nutrients · 2022Review
- Rare and intractable fibrodysplasia ossificans progressiva shows different PBMC phenotype possibly modulated by ascorbic acid and propranolol treatment.Intractable & rare diseases research · 2021Article
- Spices, Condiments, Extra Virgin Olive Oil and Aromas as Not Only Flavorings, but Precious Allies for Our Wellbeing.Antioxidants (Basel, Switzerland) · 2021Review
- Effects of Citrus Fruit Juices and Their Bioactive Components on Inflammation and Immunity: A Narrative Review.Frontiers in immunology · 2021Review
- Pyridoxine and pancreatic acinar cells: transport physiology and effect on gene expression profile.American journal of physiology. Cell physiology · 2019Article
- Propranolol and ascorbic acid in control of fibrodysplasia ossificans progressiva flare-ups due to accidental falls.Intractable & rare diseases research · 2019Article
- Vitamin C in Cancer: A Metabolomics Perspective.Frontiers in physiology · 2018Review
- Vitamin C and Immune Function.Nutrients · 2017Review
- Metabolic network rewiring of propionate flux compensates vitamin B12 deficiency in C. elegans.eLife · 2016Article
- Regulator Versus Effector Paradigm: Interleukin-10 as Indicator of the Switching Response.Clinical reviews in allergy & immunology · 2016Review
- Effects of Antioxidants in Human Cancers: Differential Effects on Non-Coding Intronic RNA Expression.Antioxidants (Basel, Switzerland) · 2016Article
- Modulation of Cytokines in Cancer Patients by Intravenous Ascorbate Therapy.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Resolution of sterile inflammation: role for vitamin C.Mediators of inflammation · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to study the effects of vitamin C supplementation on gene expression and compare its action between physiological and inflammatory conditions, a pilot study was set up utilizing microarray and qPCR technologies. Five healthy volunteers were supplemented with 1 g vitamin C (Redoxon(®)) per day for five consecutive days. Peripheral blood mononuclear cells (PBMNC) were isolated before and just after the last supplementation, and RNA was isolated for the Affymetrix gene 1.0 ST chip analysis. PBMNC were also, ex vivo, treated with LPS, and gene expression was quantified by means of a "Human NFkB Signaling" qPCR array. Only a very moderate effect on the baseline gene expression modulation was associated with vitamin C supplementation. However, in spite of the limited number of subjects analyzed, vitamin C supplementation resulted in a markedly different modulation of gene expression upon the inflammatory stimulus, specifically at the level of the MyD88-dependent pathway and of the anti-inflammatory cytokine IL-10 synthesis. This study suggests that vitamin C supplementation in healthy subjects, not selected according to a specific genetic profile, consuming an adequate amount of vitamin C, and having a satisfactory vitamin C plasma concentration at the baseline, does not result in a significant modification of gene expression profile. Under this satisfactory micronutrient status, supplementation of vitamin C is "buffered" within a homeostatic physiological equilibrium. Differently, following a second "hit" constituted of an inflammatory stimulus such as LPS, able to trigger a critical burst to the normal physiological state, the higher availability of ascorbic acid emerges, and results in a significant modulation of cell response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.