Evidence map›Paper›PMID 24603933›Full record

ArticleNature protocols2014

Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.

Bogumil J Karas, Jelena Jablanovic, Edward Irvine, Lijie Sun, Li Ma, Philip D Weyman, Daniel G Gibson, John I Glass, J Craig Venter, Clyde A Hutchison and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Selection-free whole genome transplantation revives dead microbes.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Localization ofPlant signaling & behavior · 2023
    Article
  7. Review
  8. Article
  9. Review
  10. Cross-species microbial genome transfer: a Review.Frontiers in bioengineering and biotechnology · 2023
    Review
  11. Article
  12. Review
  13. Targeting Nanodiamonds to the Nucleus in Yeast Cells.Nanomaterials (Basel, Switzerland) · 2020
    Article
  14. Article
  15. Trans-Kingdom Conjugation within Solid Media fromInternational journal of molecular sciences · 2019
    Article
  16. Article
  17. Article
  18. Engineering yeast endosymbionts as a step toward the evolution of mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Bogumil J KarasDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Jelena JablanovicDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Edward IrvineDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Lijie SunDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Li MaDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, Rockville, Maryland, USA.
Philip D WeymanDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Daniel G GibsonDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
John I GlassDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, Rockville, Maryland, USA.
J Craig Venter1] Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA. [2] Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, Rockville, Maryland, USA.
Clyde A HutchisonDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Hamilton O SmithDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.
Yo SuzukiDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Direct cell-to-cell transfer of genomes from bacteria to yeast facilitates genome engineering for bacteria that are not amenable to genetic manipulation by allowing instead for the utilization of the powerful yeast genetic tools. Here we describe a protocol for transferring whole genomes from bacterial cells to yeast spheroplasts without any DNA purification process. The method is dependent on the treatment of the bacterial and yeast cellular mixture with PEG, which induces cell fusion, engulfment, aggregation or lysis. Over 80% of the bacterial genomes transferred in this way are complete, on the basis of structural and functional tests. Excluding the time required for preparing starting cultures and for incubating cells to form final colonies, the protocol can be completed in 3 h.

Indexed as

Genome, BacterialCloning, MolecularDNAGenetic EngineeringMycoplasma mycoidesSaccharomyces cerevisiaeSpheroplastsDNA

Identifiers

PMID24603933

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.