ArticlePloS one2014
Fibroblast growth factor 9 activates akt and MAPK pathways to stimulate steroidogenesis in mouse leydig cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- FGF9 treatment reduces off-target chondrocytes from iPSC-derived kidney organoids.NPJ Regenerative medicine · 2025Article
- VEGF-dependent testicular vascularisation involves MEK1/2 signalling and the essential angiogenesis factors, SOX7 and SOX17.BMC biology · 2024Article
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- Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Animals : an open access journal from MDPI · 2022Article
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- FGF9 promotes cell proliferation and tumorigenesis in TM3 mouse Leydig progenitor cells.American journal of cancer research · 2022Article
- Identification and Characterization of lncRNA and mRNA in Testes of Landrace and Hezuo Boars.Animals : an open access journal from MDPI · 2021Article
- Cytoarchitectural improvement in Leydig cells of diabetic rats after treatment with aqueous and ethanol extracts ofJournal of traditional and complementary medicine · 2021Article
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- Genome-wide DNA methylation analysis of KRAS mutant cell lines.Scientific reports · 2020Article
- Deletion of inositol polyphosphate 4-phosphatase type-II B affects spermatogenesis in mice.PloS one · 2020Article
- Brain-derived neurotrophic factor: A steroidogenic regulator of Leydig cells.Journal of cellular physiology · 2019Article
- Propofol Inhibits Androgen Production in Rat Immature Leydig Cells.Frontiers in pharmacology · 2019Article
- Involvement of Activation of Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- FGF9/FGFR2 increase cell proliferation by activating ERK1/2, Rb/E2F1, and cell cycle pathways in mouse Leydig tumor cells.Cancer science · 2018Article
- Ameliorative effect of ginseng extract on phthalate and bisphenol A reprotoxicity during pregnancy in rats.Environmental science and pollution research international · 2018Article
- Transcriptome profiling of bovine ovarian theca cells treated with fibroblast growth factor 9.Domestic animal endocrinology · 2018Article
- Article
- Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle.Molecular and cellular endocrinology · 2017Article
- The expression profiles of fibroblast growth factor 9 and its receptors in developing mice testes.Organogenesis · 2016Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibroblast growth factor 9 (FGF9) is a multifunctional polypeptide belonging to the FGF family and has functions related to bone formation, lens-fiber differentiation, nerve development, gap-junction formation and sex determination. In a previous study, we demonstrated that FGF9 stimulates the production of testosterone in mouse Leydig cells. In the present study, we used both primary mouse Leydig cells and MA-10 mouse Leydig tumor cells to further investigate the molecular mechanism of FGF9-stimulated steroidogenesis. Results showed that FGF9 significantly activated steroidogenesis in both mouse primary and tumor Leydig cells (p<0.05). Furthermore, FGF9 significantly induced the expression of phospho-Akt at 0.5 and 24 hr, phospho-JNK at 0.25, 0.5, and 24 hr, phospho-p38 at 0.5 hr, and phospho-ERK1/2 from 0.25 to 24 hr in primary Leydig cells (p<0.05). Also, FGF9 significantly up-regulated the expression of phospho-Akt at 3 hr, phospho-JNK at 0.25 hr, and phospho-ERK1/2 at 1 and 3 hr in MA-10 cells (p<0.05). Using specific inhibitors of Akt, JNK, p38, and ERK1/2, we further demonstrated that the inhibitors of Akt and ERK1/2 significantly suppressed the stimulatory effect of FGF9 on steroidogenesis in mouse Leydig cells. In conclusion, FGF9 specifically activated the Akt and ERK1/2 in normal mouse Leydig cells and the Akt, JNK and ERK1/2 in MA-10 mouse Leydig tumor cells to stimulate steroidogenesis.
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