ArticlePLoS pathogens2014
The hypervariable amino-terminus of P1 protease modulates potyviral replication and host defense responses.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 115 citations in OpenAlex.
- Contrasting Effects of Tagging Turnip Mosaic Virus Proteins.Pathogens (Basel, Switzerland) · 2026Article
- Targeting ParPMC Susceptibility Hubs in Prunus: eEF1A as a Conserved Mediator of Plum Pox Virus Infection in Nicotiana benthamiana.Molecular plant pathology · 2026Article
- Defensin-like Protein Homologues Participate in Antiviral Resistance inLife (Basel, Switzerland) · 2026Article
- Complete genome sequence and molecular characterization of a novel potyvirus (sweet potato virus H) infecting sweet potato.Archives of virology · 2025Article
- Natural self-attenuation of pathogenic viruses by deleting the silencing suppressor coding sequence for long-term plant-virus coexistence.PLoS pathogens · 2025Article
- Cu/Zn superoxide dismutase homologs participate inFrontiers in microbiology · 2025Article
- N6-adenosine-methyltransferase MTA70-like participates antiviral responses inFrontiers in microbiology · 2025Article
- Replacement of P1 of soybean mosaic virus with P1 of clover yellow vein virus has no impact on virus viability and host specificity.Archives of virology · 2024Article
- Rubisco small subunit (RbCS) is co-opted by potyvirids as the scaffold protein in assembling a complex for viral intercellular movement.PLoS pathogens · 2024Article
- Survey, Detection, Characterization of Papaya Ringspot Virus from Southern India and Management of Papaya Ringspot Disease.Pathogens (Basel, Switzerland) · 2023Article
- Role of Plant Virus Movement Proteins in Suppression of Host RNAi Defense.International journal of molecular sciences · 2023Review
- The Mechanism of Resistance of EUROPEAN Plum toPlants (Basel, Switzerland) · 2023Article
- Mapping allosteric pathway in NIa-Pro using computational approach.Quantitative biology (Beijing, China) · 2023Article
- A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus.Journal of virology · 2023Article
- P1 of turnip mosaic virus interacts with NOD19 for vigorous infection.Frontiers in microbiology · 2023Article
- AlkB RNA demethylase homologues and NMolecular plant pathology · 2022Article
- Leek Yellow Stripe Virus Can Adjust for Host Adaptation by Trimming the N-Terminal Domain to Allow the P1 Protein to Function as an RNA Silencing Suppressor.The plant pathology journal · 2022Article
- Proteome expansion in the Potyviridae evolutionary radiation.FEMS microbiology reviews · 2022Review
- ITRAQ-Based Proteomic Analysis of Wheat (Biology · 2022Article
- Water Deficit Improves Reproductive Fitness inPlants (Basel, Switzerland) · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The replication of many RNA viruses involves the translation of polyproteins, whose processing by endopeptidases is a critical step for the release of functional subunits. P1 is the first protease encoded in plant potyvirus genomes; once activated by an as-yet-unknown host factor, it acts in cis on its own C-terminal end, hydrolyzing the P1-HCPro junction. Earlier research suggests that P1 cooperates with HCPro to inhibit host RNA silencing defenses. Using Plum pox virus as a model, we show that although P1 does not have a major direct role in RNA silencing suppression, it can indeed modulate HCPro function by its self-cleavage activity. To study P1 protease regulation, we used bioinformatic analysis and in vitro activity experiments to map the core C-terminal catalytic domain. We present evidence that the hypervariable region that precedes the protease domain is predicted as intrinsically disordered, and that it behaves as a negative regulator of P1 proteolytic activity in in vitro cleavage assays. In viral infections, removal of the P1 protease antagonistic regulator is associated with greater symptom severity, induction of salicylate-dependent pathogenesis-related proteins, and reduced viral loads. We suggest that fine modulation of a viral protease activity has evolved to keep viral amplification below host-detrimental levels, and thus to maintain higher long-term replicative capacity.
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