ArticlePloS one2014
Specificity of ε and non-ε isoforms of arabidopsis 14-3-3 proteins towards the H+-ATPase and other targets.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- 14-3-3 proteins: Central hubs in plant development and stress adaptation.Journal of integrative plant biology · 2026Review
- Genome-Wide Identification and Characterization of thePlants (Basel, Switzerland) · 2026Article
- A 14-3-3 modulator of seed weight and quality for unlocking the yield potential of soybean.Nature communications · 2025Article
- Genetic Dissection of Rice Plant-Specific Non-ε Group 14-3-3 Proteins in Response to Phosphate Starvation.Rice (New York, N.Y.) · 2025Article
- Genome-wide identification and comparative evolution of 14-3-3 gene family members in five Brassicaceae species.BMC genomics · 2025Article
- 14-3-3 Proteins and the Plasma Membrane HPlants (Basel, Switzerland) · 2023Article
- Haplotype Analysis of GmSGF14 Gene Family Reveals Its Roles in Photoperiodic Flowering and Regional Adaptation of Soybean.International journal of molecular sciences · 2023Article
- The Salt Tolerance-Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress inPlants (Basel, Switzerland) · 2023Article
- The role of 14-3-3 proteins in plant growth and response to abiotic stress.Plant cell reports · 2022Review
- Stigma/Style Cell-Cycle Inhibitor 1, a Regulator of Cell Proliferation, Interacts With a Specific 14-3-3 Protein and Is Degraded During Cell Division.Frontiers in plant science · 2022Article
- Review
- Evolution of 14-3-3 Proteins in Angiosperm Plants: Recurring Gene Duplication and Loss.Plants (Basel, Switzerland) · 2021Article
- The p53 family member p73 in the regulation of cell stress response.Biology direct · 2021Review
- Global mapping of cancers: The Cancer Genome Atlas and beyond.Molecular oncology · 2021Review
- Serine and one-carbon metabolisms bring new therapeutic venues in prostate cancer.Discover oncology · 2021Review
- Redressing the interactions between stem cells and immune system in tissue regeneration.Biology direct · 2021Review
- The Surprising Story of Fusicoccin: A Wilt-Inducing Phytotoxin, a Tool in Plant Physiology and a 14-3-3-Targeted Drug.Biomolecules · 2021Review
- The expression of ELOVL4, repressed by MYCN, defines neuroblastoma patients with good outcome.Oncogene · 2021Article
- Recent advances in cancer immunotherapy.Discover oncology · 2021Review
- Understanding p53 tumour suppressor network.Biology direct · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
14-3-3 proteins are a family of ubiquitous dimeric proteins that modulate many cellular functions in all eukaryotes by interacting with target proteins. 14-3-3s exist as a number of isoforms that in Arabidopsis identifies two major groups named ε and non-ε. Although isoform specificity has been demonstrated in many systems, the molecular basis for the selection of specific sequence contexts has not been fully clarified. In this study we have investigated isoform specificity by measuring the ability of different Arabidopsis 14-3-3 isoforms to activate the H+-ATPase. We observed that GF14 isoforms of the non-ε group were more effective than ε group isoforms in the interaction with the H+-ATPase and in the stimulation of its activity. Kinetic and thermodynamic parameters of the binding of GF14ε and GF14ω isoforms, representative of ε and non-ε groups respectively, with the H+-ATPase, have been determined by Surface Plasmon Resonance analysis demonstrating that the higher affinity of GF14ω is mainly due to slower dissociation. The role of the C-terminal region and of a Gly residue located in the loop 8 and conserved in all non-ε isoforms has also been studied by deletion and site-specific mutagenesis. The C-terminal domains, despite their high divergence, play an auto-inhibitory role in both isoforms and they, in addition to a specific residue located in the loop 8, contribute to isoform specificity. To investigate the generality of these findings, we have used the SPOT-synthesis technology to array a number of phosphopeptides matching known or predicted 14-3-3 binding sites present in a number of clients. The results of this approach confirmed isoform specificity in the recognition of several target peptides, suggesting that the isoform specificity may have an impact on the modulation of a variety of additional protein activities, as suggested by probing of a phosphopeptide array with members of the two 14-3-3 groups.
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