ArticleVaccine2014
Profiling human antibody responses by integrated single-cell analysis.
Article in Vaccine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Cell-free immuno-profiling on a genetically programmed biochip.Nature nanotechnology · 2026Article
- Article
- High-throughput strategies for monoclonal antibody screening: advances and challenges.Journal of biological engineering · 2025Review
- Measuring single-cell protein secretion in immunology: Technologies, advances, and applications.European journal of immunology · 2021Review
- Acute Lymph Node Slices Are a Functional Model System to Study Immunity Ex Vivo.ACS pharmacology & translational science · 2021Article
- Single-Cell Technologies for the Study of Antibody-Secreting Cells.Frontiers in immunology · 2021Review
- An Automated Fluorescence-Based Method to Isolate Bone Marrow-Derived Plasma Cells from Rhesus Macaques Using SIVmac239 SOSIP.664.Molecular therapy. Methods & clinical development · 2020Article
- Advances of Single-Cell Protein Analysis.Cells · 2020Review
- Single-cell analysis targeting the proteome.Nature reviews. Chemistry · 2020Review
- Single-Cell Technologies Applied to HIV-1 Research: Reaching Maturity.Frontiers in microbiology · 2020Review
- Novel vaccines: Technology and development.The Journal of allergy and clinical immunology · 2019Review
- Detection of a Low Level and Heterogeneous B Cell Immune Response in Peripheral Blood of Acute Borreliosis Patients With High Throughput Sequencing.Frontiers in immunology · 2019Article
- Two Vaccines formSphere · 2018Article
- Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells.Frontiers in immunology · 2018Article
- A method for the isolation and characterization of functional murine monoclonal antibodies by single B cell cloning.Journal of immunological methods · 2017Article
- Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo.The Analyst · 2017Article
- Article
- Generation of Long-Lived Bone Marrow Plasma Cells Secreting Antibodies Specific for the HIV-1 gp41 Membrane-Proximal External Region in the Absence of Polyreactivity.Journal of virology · 2016Article
- Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris.Biotechnology and bioengineering · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Comprehensive characterization of the antigen-specific B cells induced during infections or following vaccination would facilitate the discovery of novel antibodies and inform how interventions shape protective humoral responses. The analysis of human B cells and their antibodies has been performed using flow cytometry to evaluate memory B cells and expanded plasmablasts, while microtechnologies have also provided a useful tool to examine plasmablasts/plasma cells after vaccination. Here we present an integrated analytical platform, using arrays of subnanoliter wells (nanowells), for constructing detailed profiles for human B cells comprising the immunophenotypes of these cells, the distribution of isotypes of the secreted antibodies, the specificity and relative affinity for defined antigens, and for a subset of cells, the genes encoding the heavy and light chains. The approach combines on-chip image cytometry, microengraving, and single-cell RT-PCR. Using clinical samples from HIV-infected subjects, we demonstrate that the method can identify antigen-specific neutralizing antibodies, is compatible with both plasmablasts/plasma cells and activated memory B cells, and is well-suited for characterizing the limited numbers of B cells isolated from tissue biopsies (e.g., colon biopsies). The technology should facilitate detailed analyses of human humoral responses for evaluating vaccines and their ability to raise protective antibody responses across multiple anatomical compartments.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.