ArticleBMC microbiology2014
Amylase production by Preussia minima, a fungus of endophytic origin: optimization of fermentation conditions and analysis of fungal secretome by LC-MS.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 65 citations in OpenAlex.
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- Comparative Profiling of the Fecal Bacteriome, Mycobiome, and Protist Community in Wild Versus Captive (Animals : an open access journal from MDPI · 2025Article
- An Update of Fungal Endophyte Diversity and Strategies for Augmenting Therapeutic Potential of their Potent Metabolites: Recent Advancement.Applied biochemistry and biotechnology · 2025Review
- Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection inInternational journal of molecular sciences · 2024Article
- Insect α-Amylases and Their Application in Pest Management.Molecules (Basel, Switzerland) · 2023Review
- RNA-Seq-Based Transcriptome Analysis of Nitric Oxide Scavenging Response inJournal of fungi (Basel, Switzerland) · 2023Article
- Alkaliphilic/Alkali-Tolerant Fungi: Molecular, Biochemical, and Biotechnological Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Towards further understanding the applications of endophytes: enriched source of bioactive compounds and bio factories for nanoparticles.Frontiers in plant science · 2023Review
- Biotransformation ability of endophytic fungi: from species evolution to industrial applications.Applied microbiology and biotechnology · 2021Review
- Physiological Characteristics and Comparative Secretome Analysis ofFrontiers in microbiology · 2021Article
- Natural Fungicolous Regulators ofMicroorganisms · 2020Article
- Enzymatic hydrolysis of starch into sugars is influenced by microgel assembly.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter.Microbial cell factories · 2018Article
- Diversity and antimicrobial activity of culturable fungi from fishscale bamboo (Phyllostachys heteroclada) in China.World journal of microbiology & biotechnology · 2017Article
- Analysis on evolutionary relationship of amylases from archaea, bacteria and eukaryota.World journal of microbiology & biotechnology · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnvironmental screening programs are used to find new enzymes that may be utilized in large-scale industrial processes. Among microbial sources of new enzymes, the rationale for screening fungal endophytes as a potential source of such enzymes relates to the hypothesised mutualistic relationship between the endophyte and its host plant. There is a need for new microbial amylases that are active at low temperature and alkaline conditions as these would find industrial applications as detergents.
resultsAn α-amylase produced by Preussia minima, isolated from the Australian native plant, Eremophilia longifolia, was purified to homogeneity through fractional acetone precipitation and Sephadex G-200 gel filtration, followed by DEAE-Sepharose ion exchange chromatography. The purified α-amylase showed a molecular mass of 70 kDa which was confirmed by zymography. Temperature and pH optima were 25°C and pH 9, respectively. The enzyme was activated and stabilized mainly by the metal ions manganese and calcium. Enzyme activity was also studied using different carbon and nitrogen sources. It was observed that enzyme activity was highest (138 U/mg) with starch as the carbon source and L-asparagine as the nitrogen source. Bioreactor studies showed that enzyme activity was comparable to that obtained in shaker cultures, which encourages scale-up fermentation for enzyme production. Following in-gel digestion of the purified protein by trypsin, a 9-mer peptide was sequenced and analysed by LC-ESI-MS/MS. The partial amino acid sequence of the purified enzyme presented similarity to α-amylase from Magnaporthe oryzae.
conclusionsThe findings of the present study indicate that the purified α-amylase exhibits a number of promising properties that make it a strong candidate for application in the detergent industry. To our knowledge, this is the first amylase isolated from a Preussia minima strain of endophytic origin.
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