ArticleMolecular cytogenetics2014
An easy "SteamDrop" method for high quality plant chromosome preparation.
Article in Molecular cytogenetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 134 citations in OpenAlex.
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- Investigating the Origin and Evolution of PolyploidPlants (Basel, Switzerland) · 2023Article
- Preparation of Mitotic Chromosomes with the Dropping Technique.Methods in molecular biology (Clifton, N.J.) · 2023Article
- qPCR as a Selective Tool for Cytogenetics.Plants (Basel, Switzerland) · 2022Article
- Retention of Mutations in Colchicine-Induced Ornamental SucculentPlants (Basel, Switzerland) · 2022Article
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- Mega-sized pericentromeric blocks of simple telomeric repeats and their variants reveal patterns of chromosome evolution in ancient Cycadales genomes.The Plant journal : for cell and molecular biology · 2022Article
- Integrating Genetic and Chromosome Maps ofInternational journal of molecular sciences · 2022Article
- Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer inPlants (Basel, Switzerland) · 2022Article
- Whole genome duplication of wild-type andFrontiers in plant science · 2022Article
- A genome forHorticulture research · 2022Article
- Variation in Ribosomal DNA in the GenusPlants (Basel, Switzerland) · 2021Article
- A Dual-Color Tyr-FISH Method for Visualizing Genes/Markers on Plant Chromosomes to Create Integrated Genetic and Cytogenetic Maps.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe chromosome preparation is a crucial step for obtaining satisfactory results in molecular cytogenetic researches. The preparation of plant chromosomes for molecular cytogenetic purposes remains a challenge for some species. In contrast to human chromosome preparation, the processes occurring during plant chromosome preparation and causing chromosome spreading are still poorly understood.
resultsWe studied the dynamics of plant chromosome spreading after dropping cell suspension on slides. We showed that steam stimulates cytoplasm hydrolysis and rapid chromosome spreading and that chromosomes stretch during this chromosome spreading. Based on these observations, we developed a novel method, named "SteamDrop", for the preparation of well-spread mitotic and pachytene chromosomes and successfully used it for 28 plant species with large and small chromosomes. We applied cell suspensions in ethanol instead of the commonly used ethanol/acetic acid fixative. Mitotic and meiotic chromosomes prepared via "SteamDrop" were used in fluorescent in situ hybridization (FISH) experiments with repetitive and unique DNA probes. Long storage of cell suspensions in ethanol did not impair the quality of chromosome preparations.
conclusionThe SteamDrop procedure provides a robust and routine method for high quality plant chromosome preparations. The method can be applied for metaphase as well as pachytene chromosome preparation in wide range of species. The chromosomes prepared by SteamDrop are well suitable for repetitive and unique DNA visualization.
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