Evidence map›Paper›PMID 24600553›Full record

ArticleResults in immunology2013

Uptake of biodegradable poly(γ-glutamic acid) nanoparticles and antigen presentation by dendritic cells in vivo.

Tomofumi Uto, Masaaki Toyama, Yosuke Nishi, Takami Akagi, Fumiaki Shima, Mitsuru Akashi, Masanori Baba

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Results in immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Polymeric Nanoparticle-Based Vaccine Adjuvants and Delivery Vehicles.Current topics in microbiology and immunology · 2021
    Review
  5. Review
  6. Article
  7. Review
  8. Biodegradable polymers for modern vaccine development.Journal of industrial and engineering chemistry (Seoul, Korea) · 2019
    Review
  9. Article
  10. Article
  11. Review
  12. Micro- and nanoparticulates for DNA vaccine delivery.Experimental biology and medicine (Maywood, N.J.) · 2016
    Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tomofumi UtoDivision of Antiviral Chemotherapy, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-0544, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Masaaki ToyamaDivision of Antiviral Chemotherapy, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-0544, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Yosuke NishiDivision of Antiviral Chemotherapy, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-0544, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Takami AkagiDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Fumiaki ShimaDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Mitsuru AkashiDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.
Masanori BabaDivision of Antiviral Chemotherapy, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-0544, Japan ; Core Research for Evolutional Science and Technology (CREST), the Japan Science and Technology Agency (JST), Tokyo 150-0002, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Poly(γ-glutamic acid) (γ-PGA) nanoparticles (NPs) carrying antigens have been shown to induce potent antigen-specific immune responses. However, in vivo delivery of γ-PGA NPs to dendritic cells (DCs), a key regulator of immune responses, still remains unclear. In this study, γ-PGA NPs were examined for their uptake by DCs and subsequent migration from the skin to the regional lymph nodes (LNs) in mice. After subcutaneous injection of fluorescein 5-isothiocyanate (FITC)-labeled NPs or FITC-ovalbumin (OVA)-carrying NPs (FITC-OVA-NPs), DCs migrated from the skin to the LNs and maturated, resulting in the upregulation of the costimulatory molecules CD80 and CD86 and the chemokine receptor CCR7. However, the migrated DCs were not detected in the spleen. FITC-OVA-NPs were found to be taken up by skin-derived CD103(+) DCs, and the processed antigen peptides were cross-presented by the major histocompatibility complex (MHC) class I molecule of DCs. Furthermore, significant activation of antigen-specific CD8(+) T cells was observed in mice immunized with OVA-carrying NPs (OVA-NPs) but not with OVA alone or OVA with an aluminum adjuvant. The antigen-specific CD8(+) T cells were induced within 7 days after immunization with OVA-NPs. Thus, γ-PGA NPs carrying various antigens may have great potential as an antigen-delivery system and vaccine adjuvant in vivo.

Indexed as

Dendritic cellDrug deliveryImmune responseNanoparticleVaccine

Identifiers

PMID24600553
PMCPMC3908329

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.