ArticleFrontiers in microbiology2014
An experimental point of view on hydration/solvation in halophilic proteins.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Potassium binding by carbonyl clusters, halophilic adaptation and catalysis of Haloferax mediterranei D-2-hydroxyacid dehydrogenase.Communications biology · 2025Article
- Adapting to salinity: structural dynamics of the DNA polymerase III beta subunit in Salinibacter ruber.Extremophiles : life under extreme conditions · 2025Article
- Determination ofJournal of the Royal Society, Interface · 2025Article
- Allostery and Evolution: A Molecular Journey Through the Structural and Dynamical Landscape of an Enzyme Super Family.Molecular biology and evolution · 2025Article
- The Characterization of Ancient Methanococcales Malate Dehydrogenases Reveals That Strong Thermal Stability Prevents Unfolding Under Intense γ-Irradiation.Molecular biology and evolution · 2024Article
- Article
- Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 fromRSC sustainability · 2024Article
- Deciphering Evolutionary Trajectories of Lactate Dehydrogenases Provides New Insights into Allostery.Molecular biology and evolution · 2023Article
- Molecular evolution steered structural adaptations in the DNA polymerase III α subunit of halophilic bacterium Salinibacter ruber.Extremophiles : life under extreme conditions · 2023Article
- Experimental study of proteome halophilicity using nanoDSF: a proof of concept.Extremophiles : life under extreme conditions · 2021Article
- Calcium-Binding Generates the Semi-Clathrate Waters on a Type II Antifreeze Protein to Adsorb onto an Ice Crystal Surface.Biomolecules · 2019Article
- NewFrontiers in microbiology · 2019Article
- Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Article
- Compatible Solute Synthesis and Import by the Moderate HalophileFrontiers in microbiology · 2018Article
- Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart.The protein journal · 2017Article
- SAXS/SANS on Supercharged Proteins Reveals Residue-Specific Modifications of the Hydration Shell.Biophysical journal · 2016Article
- Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst.Nature communications · 2015Article
- Stay Wet, Stay Stable? How Internal Water Helps the Stability of Thermophilic Proteins.The journal of physical chemistry. B · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-solvent interactions govern the behaviors of proteins isolated from extreme halophiles. In this work, we compared the solvent envelopes of two orthologous tetrameric malate dehydrogenases (MalDHs) from halophilic and non-halophilic bacteria. The crystal structure of the MalDH from the non-halophilic bacterium Chloroflexus aurantiacus (Ca MalDH) solved, de novo, at 1.7 Å resolution exhibits numerous water molecules in its solvation shell. We observed that a large number of these water molecules are arranged in pentagonal polygons in the first hydration shell of Ca MalDH. Some of them are clustered in large networks, which cover non-polar amino acid surface. The crystal structure of MalDH from the extreme halophilic bacterium Salinibacter ruber (Sr) solved at 1.55 Å resolution shows that its surface is strongly enriched in acidic amino acids. The structural comparison of these two models is the first direct observation of the relative impact of acidic surface enrichment on the water structure organization between a halophilic protein and its non-adapted counterpart. The data show that surface acidic amino acids disrupt pentagonal water networks in the hydration shell. These crystallographic observations are discussed with respect to halophilic protein behaviors in solution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.