ArticleJournal of experimental botany2014
New insights into the structural and spatial variability of cell-wall polysaccharides during wheat grain development, as revealed through MALDI mass spectrometry imaging.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 79 citations in OpenAlex.
- Optimization of Ionic Matrix Deposition to Increase the Performance of MALDI-Based Spatial N-Glycomics.Journal of mass spectrometry : JMS · 2026Article
- Spatiotemporal metabolic responses to water deficit stress in distinct leaf cell-types of poplar.Frontiers in plant science · 2024Article
- Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat.Plants (Basel, Switzerland) · 2023Review
- Insights into the Functional Components in Wheat Grain: Spatial Pattern, Underlying Mechanism and Cultivation Regulation.Plants (Basel, Switzerland) · 2023Review
- Article
- Grapevine leaf MALDI-MS imaging reveals the localisation of a putatively identified sucrose metabolite associated toFrontiers in plant science · 2022Article
- Esmraldi: efficient methods for the fusion of mass spectrometry and magnetic resonance images.BMC bioinformatics · 2021Article
- Article
- Loss of TaIRX9b gene function in wheat decreases chain length and amount of arabinoxylan in grain but increases cross-linking.Plant biotechnology journal · 2020Article
- Article
- Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Metabolites during Sorghum Germination.Plant physiology · 2020Article
- Response Surface Methodology As a New Approach for Finding Optimal MALDI Matrix Spraying Parameters for Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2020Article
- Article
- Spatial distribution of functional components in the starchy endosperm of wheat grains.Journal of cereal science · 2020Review
- Cell Wall Proteome of Wheat Grain Endosperm and Outer Layers at Two Key Stages of Early Development.International journal of molecular sciences · 2019Article
- Distribution of cell wall hemicelluloses in the wheat grain endosperm: a 3D perspective.Planta · 2018Article
- Monitoring Polysaccharide Dynamics in the Plant Cell Wall.Plant physiology · 2018Review
- High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration.Plant biotechnology journal · 2017Article
- Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging.PloS one · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabinoxylans (AX) and (1→3),(1→4)-β-glucans (BG) are the major components of wheat grain cell walls. Although incompletely described at the molecular level, it is known that the chemical and distributional heterogeneity of these compounds impacts the quality and use of wheat. In this work, an emerging technique based on MALDI mass spectrometry imaging (MSI) was employed to map variations in the quantity, localization, and structure of these polysaccharides in the endosperm during wheat maturation. MALDI MSI couples detailed structural information with the spatial localization observed at the micrometer scale. The enzymic hydrolysis of AX and BG was performed directly on the grain sections, resulting in the efficient formation of smaller oligosaccharides that are easily measurable through MS, with no relocation across the grain. The relative quantification of the generated oligosaccharides was achieved. The method was validated by confirming data previously obtained using other analytical techniques. Furthermore, in situ analysis of grain cell walls through MSI revealed previously undetectable intense acetylation of AX in young compared to mature grains, together with findings concerning the feruloylation of AX and different structural features of BG. These results provide new insights into the physiological roles of these polysaccharides in cell walls and the specificity of the hydrolytic enzymes involved.
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