Evidence map›Paper›PMID 24599264›Full record

ArticlePloS one2014

Preconditioning of mesenchymal stem cells by sevoflurane to improve their therapeutic potential.

Xuejun Sun, Bo Fang, Xi Zhao, Guangwei Zhang, Hong Ma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xuejun SunDepartment of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Bo FangDepartment of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Xi ZhaoDepartment of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Guangwei ZhangDepartment of Cardiac Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
Hong MaDepartment of Anesthesiology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China.
China Medical University · CNFirst Hospital of China Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBone marrow mesenchymal stem cells (MSCs) have been found to produce beneficial effects on ischemia-reperfusion injury. However, most of the MSCs died when transplanted into the ischemic tissue, which severely limit their therapeutic potential.

methodsUsing an in vitro model of hypoxia and serum deprivation (H/SD), we investigated the hypothesis that sevoflurane preconditioning could protect MSCs against H/SD-induced apoptosis and improve their migration, proliferation, and therapeutic potential. The H/SD of MSCs and neuron-like PC12 cells were incubated in a serum-free medium and an oxygen concentration below 0.1% for 24 h. Sevoflurane preconditioning was performed through a 2-h incubation of MSCs in an airtight chamber filled with 2 vol% sevoflurane. Apoptosis of MSCs or neuron-like PC12 cells was assessed using Annexin V-FITC/propidium iodide (PI). Furthermore, the mitochondrial membrane potential was assessed using lipophilic cationic probe. The proliferation rate was evaluated through cell cycle analysis. Finally, HIF-1α, HIF-2α, VEGF and p-Akt/Akt levels were measured by western blot.

resultsSevoflurane preconditioning minimized the MSCs apoptosis and loss of mitochondrial membrane potential. Furthermore, it increased the migration and expression of HIF-1α, HIF-2α, VEGF, and p-Akt/Akt, reduced by H/SD. In addition, neuron-like PC12 cells were more resistant to H/SD-induced apoptosis when they were co-cultured with sevoflurane preconditioning MSCs.

conclusionThese findings suggest that sevoflurane preconditioning produces protective effects on survival and migration of MSCs against H/SD, as well as improving the therapeutic potential of MSCs. These beneficial effects might be mediated at least in part by upregulating HIF-1α, HIF-2α, VEGF, and p-Akt/Akt.

Indexed as

Mesenchymal Stem Cell TransplantationAnimalsApoptosisBiomarkersCell HypoxiaCell MovementCell ProliferationCell SurvivalCulture Media, Serum-FreeFlow CytometryHypoxia-Inducible Factor 1, alpha SubunitMesenchymal Stem CellsMethyl EthersPC12 CellsProto-Oncogene Proteins c-aktRatsBiomarkersCulture Media, Serum-FreeHypoxia-Inducible Factor 1, alpha SubunitMethyl EthersProto-Oncogene Proteins c-aktSevoflurane

Identifiers

PMID24599264
PMCPMC3944720
OpenAlexW2041011994

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.