ArticleMolecules and cells2014
Glucosylation of isoflavonoids in engineered Escherichia coli.
Article in Molecules and cells, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 60 citations in OpenAlex.
- Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides.Microbial biotechnology · 2025Article
- Antioxidant and Anti-Inflammatory Activities of Methanol Extract ofInternational journal of molecular sciences · 2025Article
- Biosynthesis of Apigenin Glucosides in EngineeredJournal of microbiology and biotechnology · 2024Article
- Biosynthesis and Anticancer Activity of Genistein Glycoside Derivatives.Anti-cancer agents in medicinal chemistry · 2024Review
- Genomic signals of local adaptation in Picea crassifolia.BMC plant biology · 2023Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- Plant Glycosides and Glycosidases: A Treasure-Trove for Therapeutics.Frontiers in plant science · 2020Review
- Biocatalytic Synthesis of Non-Natural MonoterpeneACS omega · 2019Article
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
- Mining of efficient microbial UDP-glycosyltransferases by motif evolution cross plant kingdom for application in biosynthesis of salidroside.Scientific reports · 2017Article
- OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation.Chembiochem : a European journal of chemical biology · 2017Article
- Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification.Scientific reports · 2016Article
- When plants produce not enough or at all: metabolic engineering of flavonoids in microbial hosts.Frontiers in plant science · 2015Review
- Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives.Applied and environmental microbiology · 2014Article
- Enzymatic glycosylation of the topical antibiotic mupirocin.Glycoconjugate journal · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A glycosyltransferase, YjiC, from Bacillus licheniformis has been used for the modification of the commercially available isoflavonoids genistein, daidzein, biochanin A and formononetin. The in vitro glycosylation reaction, using UDP-α-D-glucose as a donor for the glucose moiety and aforementioned four acceptor molecules, showed the prominent glycosylation at 4' and 7 hydroxyl groups, but not at the 5(th) hydroxyl group of the A-ring, resulting in the production of genistein 4'-O-β-D-glucoside, genistein 7-O-β-D-glucoside (genistin), genistein 4',7-O-β-D-diglucoside, biochanin A-7-O-β-D-glucoside (sissotrin), daidzein 4'-O-β-D-glucoside, daidzein 7-O-β-D-glucoside (daidzin), daidzein 4', 7-O-β-D-diglucoside, and formononetin 7-O-β-D-glucoside (ononin). The structures of all the products were elucidated using high performance liquid chromatography-photo diode array and high resolution quadrupole time-of-flight electrospray ionization mass spectrometry (HR QTOFESI/MS) analysis, and were compared with commercially available standard compounds. Significantly higher bioconversion rates of all four isoflavonoids was observed in both in vitro as well as in vivo bioconversion reactions. The in vivo fermentation of the isoflavonoids by applying engineered E. coli BL21(DE3)/ΔpgiΔzwfΔushA overexpressing phosphoglucomutase (pgm) and glucose 1-phosphate uridyltransferase (galU), along with YjiC, found more than 60% average conversion of 200 μM of supplemented isoflavonoids, without any additional UDP-α-D-glucose added in fermentation medium, which could be very beneficial to large scale industrial production of isoflavonoid glucosides.
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