ArticleAmerican journal of physiology. Renal physiology2014
Regulation of macromolecular modulators of urinary stone formation by reactive oxygen species: transcriptional study in an animal model of hyperoxaluria.
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Antiurolithic effects of medicinal plants: results of in vivo studies in rat models of calcium oxalate nephrolithiasis-a systematic review.Urolithiasis · 2021Pooled it
- From gut to kidney: microbiota modulates stone risk through inflammation-a mediated Mendelian randomization study.Mammalian genome : official journal of the International Mammalian Genome Society · 2025Article
- The significance of reactive oxygen species in the formation of calcium oxalate stones and the protective effects of antioxidants on the kidneys.Frontiers in immunology · 2025Review
- Anion exchange chromatographic fractionation of urinary proteins followed by tandem mass spectrometry identifies potential natural inhibitors of calcium oxalate stone.Computational and structural biotechnology journal · 2025Article
- Porous Se@SiOTranslational andrology and urology · 2024Article
- A spatially anchored transcriptomic atlas of the human kidney papilla identifies significant immune injury in patients with stone disease.Nature communications · 2023Article
- Proposal for pathogenesis-based treatment options to reduce calcium oxalate stone recurrence.Asian journal of urology · 2023Review
- The role of reactive oxygen species derived from different NADPH oxidase isoforms and mitochondria in oxalate-induced oxidative stress and cell injury.Urolithiasis · 2022Article
- Randall's plaque and calcium oxalate stone formation: role for immunity and inflammation.Nature reviews. Nephrology · 2021Review
- Antioxidant and Polyphenol-Rich Ethanolic Extract ofMolecules (Basel, Switzerland) · 2021Article
- Resveratrol Attenuates Oxalate-Induced Renal Oxidative Injury and Calcium Oxalate Crystal Deposition by Regulating TFEB-Induced Autophagy Pathway.Frontiers in cell and developmental biology · 2021Article
- Nox1-derived oxidative stress as a common pathogenic link between obesity and hyperoxaluria-related kidney injury.Urolithiasis · 2020Article
- Sex differences in redox homeostasis in renal disease.Redox biology · 2020Review
- Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: The Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and Oxidative Stress Markers.Journal of clinical medicine · 2020Article
- Evaluation of inactive Matrix-Gla-Protein (MGP) as a biomarker for incident and recurrent kidney stones.Journal of nephrology · 2020Article
- Analyses of long non-coding RNA and mRNA profiling using RNA sequencing in calcium oxalate monohydrate-stimulated renal tubular epithelial cells.Urolithiasis · 2019Article
- Downregulated Expression of Solute Carrier Family 26 Member 6 in NRK-52E Cells Attenuates Oxalate-Induced Intracellular Oxidative Stress.Oxidative medicine and cellular longevity · 2018Article
- Histological aspects of the "fixed-particle" model of stone formation: animal studies.Urolithiasis · 2017Review
- Article
- How do stones form? Is unification of theories on stone formation possible?Archivos espanoles de urologia · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We used an unbiased approach of gene expression profiling to determine differential gene expression of all the macromolecular modulators (MMs) considered to be involved in stone formation, in hyperoxaluric rats, with and without treatment with the NADPH oxidase inhibitor apocynin. Male rats were fed rat chow or chow supplemented with 5% wt/wt hydroxy-l-proline (HLP) with or without apocynin-supplemented water. After 28 days, rats were euthanized and their kidneys explanted. Total RNA was isolated and microarray analysis was conducted using the Illumina bead array reader. Gene ontology analysis and the pathway analyses of the genes were done using Database for Annotation, Visualization of Integrated Discovery enrichment analysis tool. Quantitative RT-PCR of selected genes was carried out to verify the microarray results. Expression of selected gene products was confirmed using immunohistochemistry. Administration of HLP led to crystal deposition. Genes encoding for fibronectin, CD 44, fetuin B, osteopontin, and matrix-gla protein were upregulated while those encoding for heavy chains of inter-alpha-inhibitor 1, 3, and 4, calgranulin B, prothrombin, and Tamm-Horsfall protein were downregulated. HLP-fed rats receiving apocynin had a significant reversal in gene expression profiles: those that were upregulated came down while those that were downregulated stepped up. Apocynin treatment resulted in near complete absence of crystals. Clearly, there are two types of MMs; one is downregulated while the other is upregulated during hyperoxaluria and crystal deposition. Apparently gene and protein expressions of known macromolecular modulators of CaOx crystallization are likely regulated by ROS produced in part through the activation of NADPH oxidase.
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